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We narrowed to 440 results for: pes;

Showing: 101 - 120 of 440 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they work best attached...tag can be purified with several different resin types and is a fairly small tag that is unlikely to perturb...Affinity tags vs. other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc....

  2. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...component for expression vectors: determines which cell types the gene is expressed in and amount of recombinant...also have selectable markers for use in other cell types. Primer Binding Site A short single-stranded DNA...wide range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene...

  4. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor, you first...presence or changing cellular conditions. Other types of biosensors To focus on a particular gene or protein...the common fluorescent detection methods and the types of experiments they are commonly used for.  Table...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...mutated version of YGOI to test the different phenotypes of the specific mutations. For these purposes...effects.  And many more! We have a variety of other types of vectors for most applications used to study gene...

  6. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ... GENIE Project New viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li...Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s AAV1 Biosensor Marianne...eligible transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub ...

  7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...profiles were then compared to that of reference cell types generated by the human fetal expression atlas. Computational...along with color coded nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF...TFs which were predicted to generate unique cell types. Of the 24 TFs they tested, 22 expressed markers...

  8. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...in weeks Figure 1: Sending out 80 FedEx envelopes in a single day -- all while maintaining social...but we're planning to soon expand to other cell types, including more physiologically-relevant systems...Getting our research in line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at...

  9. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ... study gene expression in many different tissue types! Although putting a gene of interest directly downstream...hedgehog gene can be overexpressed in several cell types by using different Gal4 drivers. It is much easier...of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed PMID...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...of course, many companies selling antibodies, microscopes, and dissection tools, but there were also people...means of delivering genes to many different cell types with minimal immunological effects. AAVs are used...in experiments targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively...

  11. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... liver, we recommend using one of the natural serotypes such as AAV9 or AAV8. Very recently an engineered...collaboration injected 1e+13 vg per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that...transfer PHP.eB's enhanced CNS tropism to other serotypes, but reproducible success remains elusive.  There...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...simultaneously to monitor or control different cell types or signals in the brain. For this to work, each ...researchers can image multiple brain regions or cell types at once using existing indicators. For example, ... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...and converted that signal into a readable value. Types of biosensors So now that we know what makes up ...AAVs make it possible. In AAV systems, different serotypes can be used to affect or determine viral tropism—the...your biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous...

  14. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ... Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many viruses out there, ...tropism of AAV to target different tissue or cell types. AAV also requires some back up from adenovirus ...production. Fun fact: There are 57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5...

  15. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...nonspecific binding. Flow specific controls The three types of flow cytometry controls we’ll discuss are single... later gates, are more likely to require FMOs. Isotypes Isotype controls are negative controls that allow...Controls like single color controls, FMOs, and isotypes can help you more easily gate your target population...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on a particular...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...solute channels typically expressed on other cell types can compensate for or bypass the dysfunction of ...signaling pathways and how they lead to neurological phenotypes observed.” Using genetically-encoded biosensors...neurons from patients with GNB1 mutations, the lab hopes to capture the signalosome of different receptors...

  18. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...cx43 GJB1 GJB2 Bioelectric states can alter cell types and patterning There is mounting evidence that signaling... certain patterning activities of entire tissue types or even organs. In frogs, ectopic expression of ...even lead to the formation of whole eyes from cell types that don’t typically give rise to eyes (Pai et al...

  19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...variable gene expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature...expression. Because pSC101 is not representative of all types of plasmids, the stabilized promoters were also ...how the stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly...

  20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... sequences available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl chemical rescue of ...These diseases are characterized by overgrowth phenotypes and neural developmental delay. Mutations in ...in loss of function or dominant negative NFIX phenotypes. Developing a rapid test for the functional ...
Showing: 101 - 120 of 440 results