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We narrowed to 188 results for: pme i

Showing: 101 - 120 of 188 results

  1. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...how does NgAgo stack up thus far? A few weeks ago, I was going through NgAgo google forums where people... dig more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had...people are having difficulty with the NgAgo system. I encourage you to share your results and protocols ...More time is required for its optimization and development before it can truly be pitted against CRISPR ...Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...invisible information into actionable signals. Let's say I wanted to detect every primary metabolite in a cell...sensor domains for everything you could imagine. That I think is the next priority; we need the keyboards ... programming language is put into practice? Endy: I think it's important to understand that these platforms...with a publication in Science describing the development of “transcriptors,” transistor-like digital genetic...engineered RNA switches, but we've not yet seen the development of enough sensor domains. Ultimately, we'll need...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...successfully introduce Cre into cells of interest. What if I need two separate recombination events? One potential... 1. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. Sternberg, N. and...cell or tissue types, during certain stages of development, or by making the Cre inducible (such as with...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone from the...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... and experimental limitations and considerations? I recently spent two months in the lab of Antonello ...behavioral experiments. I’m not an electrophysiologist so I cannot speak directly on this topic but, in numerous...neural pathways involved in addiction (such as the lab I work in), an obvious behavioral experiment to test...injections until they are accurate and reproducible. I also highly recommend spending the money on a high... one of the key scientists who pioneered the development of optogenetics, has genetically manipulated ...nrn3171. PMID: 22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review...

  6. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...effort to collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...FP spectra (and rapidly growing, with your help!), I believe this represents one of the largest available...for future development of FPbase FPbase is still very much in the early stages of development, and there...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal...9E10 antibody remains a trusty antibody partner; as I write this, there are close to 10,000 citations for...0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10... mysterious, microscopic world of cells. The development of antibodies as tools allowed us to detect and...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...writing up your research? What type of journal should I choose? Writing a research paper for publication in...to include in your manuscript. What information do I need to include in my paper? More and more journals...post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes ...figures and results. This is a relatively new development, and it allows reviewers and other researchers...You to Our Guest Blogger! Maaike Pols is a Developmental Editor for F1000Research, responsible for assessing...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ..., and Dr. Angela Lin got me interested in TS when I was a newly independent faculty member studying sex...partners can regulate ion channels in the brain.  “I have a long standing interest in the biology of GPCRs...Deficiency Syndromes (CCDS) that present as global developmental delays or autism spectrum disorder. It is estimated...creatine deficiency, which is characterized by development delays, speech delays, seizures, and other symptoms... enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection...Though there are IL-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding...in Turner syndrome Turner syndrome (TS) is a developmental disorder where one of the X chromosomes is partially...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of... Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & ...Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank... Allam. Continued training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...of students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I...and collaboration. One module currently under development comes from the Caltech lab of Professor Richard... internships. A summer teacher professional development program that trains nearly 100 teachers each ...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...AAVs that have successful transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte...immune suppression to allow AAV re-administration but I do not think the field has settled on ‘best practices...Re-administration is a challenge for AAV due to the development of neutralizing antibodies in animal models. ...design in remarkably creative ways. The continued development of more and smaller CRISPR tools is an exciting...

  13. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...the UN describes. In honor of the Camelid family, I wanted to take a moment to revisit some of the ways...discovery of heavy-chain only antibodies in a camel. And I suspect we will only continue to see their impact ...sdAbs come from? In the early years of sdAbs development, the process started with immunizing a camelid...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ... for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...deadlines. Agree on a way to review project progress. I recommend putting a regular check-in meeting on the...Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website Are You...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...all over the world. When I was at the 22nd International C. elegans meeting, I was again reminded of the...zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire...computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid tools...PMC6576255. Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41...conditional protein depletion in C. elegans." Development 142.24 (2015): 4374-4384. PubMed PMID: 26552885...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent paper...editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple and effective...

  18. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...recent paper to see the Gal4/UAS system in action (5). I use the Gal4/UAS system to knock down gene expression...2015). PubMed PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress...cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed PMID: 8223268. 2. ...

  19. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists...transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired integration...as basic as survival. For those interested in development, organs of interest, such as the intestines, ...

  20. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...that will be treated in this series of posts are (i) brightness, (ii) photostability and (iii) aggregation...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...check all possible combinations of biology and equipment. Because the performance of a fluorescent protein...
Showing: 101 - 120 of 188 results