We narrowed to 191 results for: pme i

...how does NgAgo stack up thus far? A few weeks ago, I was going through NgAgo google forums where people... dig more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had...people are having difficulty with the NgAgo system. I encourage you to share your results and protocols ...More time is required for its optimization and development before it can truly be pitted against CRISPR ...Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering...

...invisible information into actionable signals. Let's say I wanted to detect every primary metabolite in a cell...sensor domains for everything you could imagine. That I think is the next priority; we need the keyboards ... programming language is put into practice? Endy: I think it's important to understand that these platforms...with a publication in Science describing the development of “transcriptors,” transistor-like digital genetic...engineered RNA switches, but we've not yet seen the development of enough sensor domains. Ultimately, we'll need...

...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...successfully introduce Cre into cells of interest. What if I need two separate recombination events? One potential... 1. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. Sternberg, N. and...cell or tissue types, during certain stages of development, or by making the Cre inducible (such as with...

...stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone from the...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...

... and experimental limitations and considerations? I recently spent two months in the lab of Antonello ...behavioral experiments. I’m not an electrophysiologist so I cannot speak directly on this topic but, in numerous...neural pathways involved in addiction (such as the lab I work in), an obvious behavioral experiment to test...injections until they are accurate and reproducible. I also highly recommend spending the money on a high... one of the key scientists who pioneered the development of optogenetics, has genetically manipulated ...nrn3171. PMID: 22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review...

...effort to collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...FP spectra (and rapidly growing, with your help!), I believe this represents one of the largest available...for future development of FPbase FPbase is still very much in the early stages of development, and there...

...step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal...9E10 antibody remains a trusty antibody partner; as I write this, there are close to 10,000 citations for...0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10... mysterious, microscopic world of cells. The development of antibodies as tools allowed us to detect and...

...writing up your research? What type of journal should I choose? Writing a research paper for publication in...to include in your manuscript. What information do I need to include in my paper? More and more journals...post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes ...figures and results. This is a relatively new development, and it allows reviewers and other researchers...You to Our Guest Blogger! Maaike Pols is a Developmental Editor for F1000Research, responsible for assessing...

..., and Dr. Angela Lin got me interested in TS when I was a newly independent faculty member studying sex...partners can regulate ion channels in the brain.  “I have a long standing interest in the biology of GPCRs...Deficiency Syndromes (CCDS) that present as global developmental delays or autism spectrum disorder. It is estimated...creatine deficiency, which is characterized by development delays, speech delays, seizures, and other symptoms... enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection...Though there are IL-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding...in Turner syndrome Turner syndrome (TS) is a developmental disorder where one of the X chromosomes is partially...

...efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of... Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & ...Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank... Allam. Continued training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional...

...AAVs that have successful transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte...immune suppression to allow AAV re-administration but I do not think the field has settled on ‘best practices...Re-administration is a challenge for AAV due to the development of neutralizing antibodies in animal models. ...design in remarkably creative ways. The continued development of more and smaller CRISPR tools is an exciting...

...of students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I...and collaboration. One module currently under development comes from the Caltech lab of Professor Richard... internships. A summer teacher professional development program that trains nearly 100 teachers each ...

...the UN describes. In honor of the Camelid family, I wanted to take a moment to revisit some of the ways...discovery of heavy-chain only antibodies in a camel. And I suspect we will only continue to see their impact ...sdAbs come from? In the early years of sdAbs development, the process started with immunizing a camelid...

... for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...deadlines. Agree on a way to review project progress. I recommend putting a regular check-in meeting on the...Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website Are You...

...all over the world. When I was at the 22nd International C. elegans meeting, I was again reminded of the...zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire...computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid tools...PMC6576255. Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41...conditional protein depletion in C. elegans." Development 142.24 (2015): 4374-4384. PubMed PMID: 26552885...

...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent paper...editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple and effective...

...Addgene to accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists... References and Resources References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari...link between COVID-19 disease severity and the development of neutralizing antibody responses in patients...

...recent paper to see the Gal4/UAS system in action (5). I use the Gal4/UAS system to knock down gene expression...2015). PubMed PMID: 21356876. 5. J. Chow, Z. Marka, I. Bartos, S. Marka, J. C. Kagan, Environmental Stress...cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed PMID: 8223268. 2. ...

...with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists...transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired integration...as basic as survival. For those interested in development, organs of interest, such as the intestines, ...

...that will be treated in this series of posts are (i) brightness, (ii) photostability and (iii) aggregation...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...check all possible combinations of biology and equipment. Because the performance of a fluorescent protein...