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We narrowed to 286 results for: prep

Showing: 101 - 120 of 286 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... minutes. Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga...purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product...Product: plasmid DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ....0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification of DNA from agarose...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often... for ddPCR set-up. Prepare the master mix in a separate area from where you prepare your sample dilutions...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...subset of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...doi: 10.1038/s41592-023-01880-5. PMID: 37188954. Preprint available on bioRxiv – doi: 10.1101/2023.03.09.531939...doi: 10.1016/j.cell.2023.05.044. PMID: 37385249. Preprint available on bioRxiv – doi: 10.1101/2023.02.07.527548...doi: 10.1038/s41587-022-01524-7. PMID: 36593408. Preprint on bioRxiv — doi: 10.1101/2021.10.13.463862.  ...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes - all...The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture, we prepare three sets...sample is barcoded, the lab team takes over and prepares the sample for entry into Addgene’s repository...is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at each stage. The...Addgene Get help with plasmid transformation, preparation, and analysis from our protocols and molecular...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is to lyse your...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...

  8. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...also be time-consuming, depending on the library prep and instrumentation used.  Here at Addgene, we sequence...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...

  12. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...Taylor et al., 2017). However, because purified AAV preps contain similar and low levels of background protein...vector preparations. Addgene has used this method to generally validate our viral vector preparation protocol...al., 2010). The specific infectivity of viral preparations is defined by the ratio of physical viral particles...viral capsids in representative viral vector preparations are genome-containing (Figure 1).    ...Electron micrograph of an Addgene AAV viral vector preparation after negative staining. Empty vector particles...viral service, we currently titrate AAV vector preparations by qPCR. To ensure accurate and reliable results...

  13. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...product (see figure). Template A high purity plasmid prep significantly increases the success rate of site...’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ... auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As...) General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression vector cannot have p15A origin...the strain. Conversely, other strains such as M15(pREP4) use a lac repressor to act directly on the expression...

  15. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13...cloning, routine cloning, cloning unstable DNA, preparing unmethylated DNA, and more. Many mutations that...Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut... dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut...restriction enzymes to cut the plasmid after preparation. rpsL thr leu thi lacY galK galT ara tonA tsx...

  16. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...removes, not just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. ...LY6C1) Stanton et al., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated...
Showing: 101 - 120 of 286 results