...Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas...

...Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves, she does...

... does have one special tip for anyone looking to try this at home in their own lab: be very careful with...

... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties...

... knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on.    Flow cytometry In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this blog post will ...

...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...

...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

...entire series as it is published. For more reading try The Discipline of Teams by Jon R. Katzenbach and ...

...freezer. No more issues deciphering handwriting or trying to find to where the binder was moved. You’ll have...and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

...unpublished evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately...likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part of good... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....recognized by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant...epitopes of this protein.   You perform flow cytometry using both antibodies, as well as a third unrelated...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

...secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions or...are always new and improved variations you could try. We hope this post helped you start planning for ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...antibodies available at Addgene Protocols for Immunocytochemistry Protocols for other antibody applications...

...or two hanging out around the lab and especially try to attend a lab meeting. Observe how the lab members...scientific meetings (and present)? Making the decision Try to rotate or interview in more than one lab when ...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...the publication record show evidence of industry collaboration? If you are considering a career at a small...

...highlighted in red above. When searching, it is worth trying alternate names, particularly for gene names. If...

...in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

...Your Science Why Scientists Should Give Twitter a Try ...

...service of COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. ...

...plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility in a few other...

...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...