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We narrowed to 456 results for: val

Showing: 101 - 120 of 456 results

  1. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...inaccuracies as they find them (anyone can register with a valid email address).  One may also simply attach important...frequently) for the sake of comparison or ease of retrieval.  These collection pages can be kept private, ... applications, a REST API allows programmatic retrieval of the majority of information in the database...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...all this information makes your manuscript more valuable to the research community. It also makes it more...research more reproducible and your results more valuable. Some journals, including F1000Research, also ...provide on data, materials, and methods, the more valuable your paper will be to the scientific community...

  4. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...-15 kD but can have weak signals due to their monovalent nature. They are stable up to 80°C and can refold... affinity reagents, trendy as that may sound. Validated antibodies are still an excellent option for many... Exhibit Improved Tumor Lysis When Placed in a Divalent Tumor Antigen Binding Format. Journal of Biological...

  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ..., to talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you...applies: what can go wrong, will go wrong. The trash removal service slams dumpsters outside the open windows...or present at lab meetings, you’ve developed a valuable skill set that will be useful in nearly any other...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...problem solving that I did in the lab has been invaluable in my more recent positions outside of the lab...how scientists can develop and highlight these valuable skills. Hopefully, having a better sense for what... you understand it. Step Two: Come up with and evaluate potential strategies to resolve the problem.  ...

  7. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...2) Public engagement - Participate in science festivals and outreach If you are comfortable talking in...in public, volunteer at science festivals. Or engage with non-scientists in an unusual setting: Discuss...making videos, presenting in pubs and at science festivals. Science communication is my way of living out...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control  In...propagate your plasmids in: DH5α, NEB Stable, ccdB Survival, Pir1, or other. Whenever possible, we propagate...gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids that contain the R6Kγ origin...

  9. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...job well done. Your report will (needs to) feel valued. Talk to your manager and find a mentor Your manager...mentor - someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your...The latter will make you feel discouraged and not valued which will undoubtedly negatively impact your work...

  10. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...and of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...transcriptional and epigenetic regulation have been validated with these proteins. Furthermore, among those ...Addgene.org Check Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR...

  12. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...created your fusion protein, it's important to validate its function relative to the untagged, wild-type...wild-type protein. You may, for example, validate that the fusion protein localizes properly by comparing the..., and John H. McDonald. "A practical guide to evaluating colocalization in biological microscopy." American...

  13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...with a value of “0” indicating those markers are completely distinct from one another, and a value of “1...completely indistinguishable. This tool is of particular value when designing a panel.     Figure 3: Similarity...

  14. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... that, when expressed, normally encourage cell survival, growth and proliferation, while tumor suppressors... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to...

  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...some items vary, for example, plasmid kits are avaliable for less than than it would cost to buy each plasmid...DH5a, but others may require strains such as ccdB survival (for Gateway plasmids).  We also like to store...plasmid, and which experimental systems it was validated in.   Please keep in mind that all of the information...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...peptides instead of an IRES when stoichiometrically equivalent levels of expression are required, we should ...construct that expresses multiple unique genes at equivalent levels upstream of an IRES fluorescent reporter...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... disease and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...manufacturing of viral vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools ...

  18. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...proteins. Cross-linking is a chemical reaction that covalently joins two molecules and it is great for preserving...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...experiences and that it often provides incredibly valuable insight into one’s work. As a community, we must...are designed specifically to allow scientists to evaluate how small changes in vector administration can...enormous amount of data potentially available to evaluate AAV vector performance. Yet, most of the technical...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... to share these invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR...
Showing: 101 - 120 of 456 results