We narrowed to 821 results for: LAS

...drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine...achieved by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how ...achieved using lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple...

... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...raising the threshold for signaling. The two main classes of receptors currently used in chemogenetics are...viral preps for your research Find chemogenetics plasmids for your research ...

...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...the full amount of time, every time. Imaging The last step in a western blot tends to be the most straightforward...

...large-scale prime editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab...sciadv.aax9249 Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante....1038/s41587-022-01613-7 Mathis, N., Allam, A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L...

...Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis...amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence of an insert...

...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...

...neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced BDNF levels...prefrontal cortex Drd1 neurons produces rapid and long-lasting antidepressant effects." Nature communications ...Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in addiction....

...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...collection Read our molecular biology reference Find plasmids for your research ...

...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...Cichlids are 2,000 fish species that evolved in the last 10,000 years (which is considered recent). They ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...

...profile problems surrounding cell culture over the last few decades some pharmacologists have tended to ... information in your lab book and on the actual flasks of cells in the incubator). This means any other...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...