We narrowed to 772 results for: INA

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...did not affect the function or efficiency of the original APEX (Lam et al. 2014). They named this mutant... technique with proximity labelling to validate binary protein-protein interaction as well as identify...

2. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ...

3. Technologies Enabled by NanoLuc® Luciferase

   Type

   Blog Post

   Published

   Feb. 8, 2018, 12:17 p.m.

   ... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

4. Finding Your Science Policy Path

   Type

   Blog Post

   Published

   Sept. 22, 2020, 1:15 p.m.

   ...with early career science policy groups, attend webinars, and learn more about the field.  Read the CCST...accelerator for some hands-on guidance.   Check out my webinar via the NC Science Policy student group SciPolPack...

5. Sequencing Options for CRISPR Genotyping

   Type

   Blog Post

   Published

   Oct. 4, 2016, 2:30 p.m.

   ...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al....

6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex...the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching for error-free...

7. Custom CRISPR Screens & the Green Listed Software

   Type

   Blog Post

   Published

   July 11, 2017, 2:30 p.m.

   ...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified.   Run your screen! Final note I am very grateful to the researchers letting...

8. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   Published

   July 20, 2021, 1:15 p.m.

   ...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise...

9. Typing CRISPR Systems

   Type

   Blog Post

   Published

   March 18, 2025, 1:15 p.m.

   ...The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing...

10. New Coomassie Protocol Video

    Type

    Blog Post

    Published

    Oct. 31, 2023, 1:15 p.m.

    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...