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We narrowed to 821 results for: LAS

Showing: 601 - 610 of 821 results
  1. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...-time, and have received a research grant in the last five years as polled in the American Association... funding for science has been decreasing for the last five years and the first federal budget request ...March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student edition...
  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...
  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...receptor (AAVR; not strictly required)2 Human fibroblast growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1...pseudotypes are described with two numbers separated by a slash: the first number refers to the genome, and the ...Request Browse all in-stock AAV preps Browse all AAV plasmids ...
  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors to consider...using optogenetic tools Once you’ve selected the class of tool appropriate for your experiment, you’ll ... https://doi.org/10.1038/nmeth.1808 Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold...
  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...cellular compartments such as the mitochondria, cytoplasm and nucleus.  We hope that this blog has provided...
  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward the cell...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...
  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... a few gRNAs suggested to be effective by world class CRISPR labs.  Check Out This Video to See How Green...particular, one slide of the presentation made a lasting impression on me. The slide showed a complex signaling...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...
  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ... models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible ... (level of zoom, intensity, possible excitation lasers) it is also important to optimize your activation...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...
  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...mitochondria, and different dyes can result in varying cytoplasmic background signals when used with the same protein...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...Self-Labeling Protein Tags Fluorescent protein plasmids and resources A practical approach to choosing...
  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... making fiber optic cables only to hook up your lasers and not have the proper controls or a proper behavioral...surgeries. Identify coordinates through a rodent brain atlas or research papers and practice with die injection...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...
Showing: 601 - 610 of 821 results