We narrowed to 814 results for: LAS
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TypeBlog PostPublishedApril 25, 2017, 2:30 p.m.... models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible ... (level of zoom, intensity, possible excitation lasers) it is also important to optimize your activation...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...
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Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m.... a few gRNAs suggested to be effective by world class CRISPR labs. Check Out This Video to See How Green...particular, one slide of the presentation made a lasting impression on me. The slide showed a complex signaling...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m.... making fiber optic cables only to hook up your lasers and not have the proper controls or a proper behavioral...surgeries. Identify coordinates through a rodent brain atlas or research papers and practice with die injection...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...2021). TGFβ1 induces resistance of human lung myofibroblasts to cell death via down-regulation of TRPA1 ... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex... -
It’s CRISPR Week at Addgene!
TypeBlog PostPublishedMay 13, 2019, 12:01 p.m....received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that ... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community of ...2017 #Deckthelab winners. We hope everyone had a blast decorating and creating memories with their labmates... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m....trying. When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m....Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution... -
New Educational Resource: The Addgene Videos Page
TypeBlog PostPublishedJune 16, 2017, 2 p.m....topic or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...