We narrowed to 884 results for: ica

... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-...

... Writing schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for...app lets you track habits, like writing. It’s basically the equivalent of crossing off days on the calendar...suggestions Hemmngway makes (it wasn’t designed for technical writing), it’s great at flagging passive voice...jargon, this tool can help you avoid overusing technical terms when writing for a general audience. The...goal of the phrase, such as being cautious, being critical, or defining terms. The phrases in Phrasebank ...

...imaging apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...

...particle to infect a cell, it has to come into physical contact with that cell. Since there is a limited... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...AAV-mediated gene expression in a target cell. Practical considerations If you have high quality AAV, you...Integration. Molecular therapy : the journal of the American Society of Gene Therapy. 2008;16(7):1189-1199.... Protocols for Work with Virus Watch Our AAV Purification Video ...

...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...backgrounds.” This work outlines the need for clear communication, fair credit through authorship, and acceptance...remotely. The authors stress the fact that “miscommunication, a lack of leadership, and inappropriate tools...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...setting. Check out the blog post “Fostering Communication in Remote Collaborations” to get more ideas....

... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...environment for research is totally dependent on communication amongst the team so getting everyone talking...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...

...remotely as an Addgene Science Communication Intern. Developing science communication skills, while also learning...panel about “Social Media for Science Communication” at the American Society for Cell Biology (ASCB) 2018...Twitter Learn about using Reddit for science communication ...

...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...directions. I would like to thank them for their time, dedication, advice and encouragement. Why virus? Why did...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....the impression that it took a lot of work and dedication to do this well ... because it did. So finally...

... the steps of gel purification and in vitro recombination. Across all modifications described in Trehan...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM...mutations, large deletions/insertions require complicated, often costly in vitro assembly methods. A new...recombination machinery, which facilitates easy genetic modification by homologous recombination. In most recombineering...

...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...distinct effector domains that work together synergistically, modeled after natural transcription activation...demonstrated in a paper in Cell that they could typically achieve 90-99% knockdown with minimal off-target...more about the rules.” He said that there is no theoretical limit on the number of genes that can be manipulated...