...time for me to complete an online social media certification on social media data, and I was given a fantastic... occasional TikTok. I was also responsible for typical entry level tasks such as stuffing new depositor...connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene have become... at Northeastern and Addgene co-op studying communications, marketing, and global fashion studies. She...

...service organization like the Red Cross or the American Cancer Society, or maybe you think about a local...services we provide like quality control, curation, technical service and open-access educational resources....decisions at Addgene If you’re ever curious what specifically drives the decision making process at Addgene...Gizela, Henry, Ina, Inna, Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly...

...symposia on Sunday afternoon were dedicated to science communication through art. Addgene Advisory Board...page and, just a few weeks ago, we attended the American Society for Microbiology's annual meeting (ASM... Jessica Welch...

... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...

... lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura, a professor...in @nchembio Title: Bye bye L-arabinose drive. Dedicated to Edo, Armin and @KhammashLab. I hope you all...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...

...preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...Roth’s lab are already available allowing you to chemically control neuronal activity while studying your...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ... service as we continue to develop it. More communication and sharing will only help research advance....

...microorganisms based on metagenomic data. MerMAIDs (Metagenomically discovered Marine, Anion-conducting and Intensely...publicly available metagenomic datasets with biophysical studies and the potential of mining datasets ...plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...CIBN), which they call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these ...

...physiology. Ribosome profiling Ribosome profiling, a biochemical method developed in 2009, takes a “snapshot” ...translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating RNA...development, the osk-TRICK reporter was double labeled, indicating translational repression. During later stages...studying translation in various cell types and physiological states. References 1. Ingolia, Nicholas T.,...

...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...Chavez, researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...particular, the Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench...

...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...