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  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...for an undergrad taking their first upper-level class. Even so, it’s quite easy (and common!) to be blind... can understand and process your information. A class of three year olds is unlikely to be able to follow...

  5. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed of four... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...contain two numerical designations, separated by a slash, that indicate the ITR and serotype, respectively...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they will be recognized...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...catchy, but beware. A distracting background or clashing colors can take away from what should be the focus...single-color background but want to add a bit more “flash,” you can include a subtle gradient (as is shown...
Showing: 651 - 660 of 808 results