...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow... and correlating them with tumor mutations and clinical responses. This platform has the potential to ...the use of optogenetic tools is expanding to immunological research. Here the Zhou group presented Opto-CRAC... et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer cell 30.1 (2016):...

... light-mediated? The researchers split the proteolytically active light chain of BoNT/B into two fragments...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...rely heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance,...the field. Arlene Sharpe’s group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9...

...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...notice the “V2.0” at the end of this plasmid which indicates that there was a prior version of this plasmid...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...generation lentiviral backbone that must be used with lentiCas9-Blast or with cell lines already expressing Cas9...

...of interest and, for in vivo work, generate a dedicated transgenic line. Using zebrafish as a model organism... for example).  Given that the liver is an immunologically active organ, this advance is likely to afford...off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP...clonal multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent...

...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules... to optogenetics but instead of light, these genetically modified receptors are activated by small molecules...some GPCRs further to respond to specific, pharmacologically-inert, small molecules. These new receptors...Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled receptor genes from the...

...α-complementation. The system described above was put to practical use in the following way. Scientists engineered...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR... Jessica Welch...

...missing or damaged nucleotides that are common to biological sources of DSBs, and which need to be processed...generated in the course of repair by NHEJ are typically small (1-10 bp) but extremely heterogeneous. There.... NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank...Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet...This is a chance to practice leadership and communication skills. The leader also provides materials such...present formally often enough) and give honest, critical feedback for improvement. Go to a science or career...

...produce genetically modified cells in less than a month's time. Addgene: What are the implications of this...Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build a DNA-binding...diseases. We are knocking out genes that have been implicated in disease or, in cases where particular variants...