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We narrowed to 808 results for: LAS

Showing: 681 - 690 of 808 results

  1. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice from ... copied below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...high affinity to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  3. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...ideally be around 5 to 20 bp away from the cut site. Lastly, if a heterozygous event is desired but only guide...Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages Find Validated...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...the three αV integrins expressed on the BJ-5ta fibroblast cells, integrin αVβ1 was critical in initiating... readily accessible recombinant antibodies and plasmids. To encourage collaboration and education in protein...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...and can include: equipment, reagents, animals, glassware, and even people. When developing a budget, I ...reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...fibrillary associated protein (GFAP), or tyrosine-hydroxylase (TH), can limit which cells express your gene...three partial sequences of the human tyrosine hydroxylase promoter in vivo. Molecular Therapy - Methods...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...targets. Or, it might be sufficient to describe or classify the labeled structures visually; for example, ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch...Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus...
Showing: 681 - 690 of 808 results