We narrowed to 611 results for: STO;

...their CPP system to study protein transduction, D’Astolfo et al. made the surprising discovery that both...NDSB-201) were required for protein delivery. D’Astolfo et al. coined the term iTOP (induced transduction...that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series of primary cell...resulting in GFP expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP...Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see if iTOP would be compatible...cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances, the potential...Efficient Intracellular Delivery of Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers ...

... maintain many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington...start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle...genetic crosses. More importantly, this knowledge will stop you from embarking on a fruitless journey of trying...information about gene function, while others offer fly stocks and other reagents. If you’re ever confused about...where the gene is expressed within the fly, whether stocks of mutant flies exist, whether any phenotypes have...mentioned the gene. The Bloomington Drosophila Stock Center at Indiana University is another tremendous...

...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources. As editor...picked our viral vector service as one of those stories. Viral vectors are viruses engineered to be safely...available and number of requests tell only part of the story. Viral vectors are, after all, a delivery method...blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the ...primary literature, soliciting information from requestors and depositors, and identifying trends in our...actually show you if the vector was useful for the requestors or how it performed. Addgene launched our AAV...platform for the sharing of that exact data. Here, requestors can upload their experimental data in a standardized...

...norvegicus genes! Mexican axolotl (Ambystoma mexicanum) Ambystoma mexicanum, aka the axolotl. Image...are five more model organisms that have left a historical impact on the scientific community. (And, check...  The E. coli genome has an extensive research history and characterization. Many proteins and processes...organisms in the world. With its extensive scientific history, highly characterized genome, and widespread utilization...organisms is its thoroughly developed and defined history in research (Krämer, 2015). As a result, a vast... https://doi.org/10.1159/000490898 Gibbs, R., Weinstock, G., Metzker, M. et al. Genome sequence of the...

...copies of a tRNA that specifically recognizes amber stop codons (TGA) to encode phosphoserine, the Sep-tRNA... the phosphorserine OTS recognizes the TAG amber stop codon, using the OTS in an unmodified bacterial ...would result in read-through translation of many stop codons and detrimental effects on cell growth. The...was genomically recoded to eliminate all 321 amber stop codons (TAG), replacing them with TAA (7). This ...TAG codon should encode phosphoserine instead of a stop codon, which will allow full length GFP to be translated...can also be cloned with an ORF of interest for customized experiments. Once the ORF of interest is cloned...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...

...negative” data We all want to be able to tell a good story when presenting scientific findings. This makes ...of information and, from a SciComm perspective, stories with links of causation from initial hypothesis...validation are easier to understand. The need for great stories has, however, made it difficult to publish so-called...original hypotheses. Looking for novelty in our stories, we are additionally averse to producing papers...published results. This over-reliance on the perfect story for a “high impact” publication can lead to the ...the tested hypothesis is correct (a great success story) even though the majority of the unpublished evidence...generate can, however, bias us. We’ve all heard stories and read papers wherein a researcher tries 5 separate...

...individuals through stock or investor pay outs. Addgene has no investors or stock. All of our proceeds...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

... chose the stop motion format for our videos as it is the most approachable for LEGOs. Stop motion consists...pictures together to form the full movement. Producing stop motion videos is very time consuming; producing ...toys” gaining life and working as scientists in a custom made, colorful laboratory. When we started, the...child to work. If you have an odd or quirky lab story you’d like to see portrayed in LEGO form, feel free...us at [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest...

...research project must have innovative yet attainable milestones that contribute to intellectual thought and discovery... The research project also serves as a stepping stone into the next professional stage of your career ... and skills do you need to use to reach these milestones? Can you get assistance from people who have ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...Think about what your personal goals are and set milestones along the way so that you don't get stuck doing... Passion fuels the drive to continue persuing milestones and working hard. Being patient, being selective...

...Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for...microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...Brainbow was inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins... such vector-based gene delivery systems, the stochastic expression of XFPs is possible by simply introducing...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...