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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 402 results for: LARGE;

Showing: 121 - 140 of 402 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Reddit. Here are some reasons why: 1. Reddit has a large and active community of people interested in science... moderators did not delete or suppress were the large number of scientist posters with serious career ...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... to its efficient use in screening experiments; large amounts of virus are required to ensure that you...

  4. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...open-access. Though it is time for me to refill my large coffee cup and get back to our booth, I will have...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...colonies that are comprised of cells with a large nucleus, large nucleoli, and little cytoplasm. Positive...other methods. This could be a reflection of the large size of the plasmid or silencing of the plasmid ...oncogenes, such as Myc. Viral vectors also have the largest genomic footprint of iPSC generating methods due...infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral...transfection efficiency is cell-type dependent and larger plasmids have lower rates of transfection. Minicircles...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

  10. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

  12. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already...CRISPR/Cas9. You check the mutation and it causes a large deletion that removes the entire exon that codes...

  13. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... and typically takes less than an hour. However large proteins typically do not transfer as well using...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence appears to ...lifespan. This work should be done in mouse and large animal models with mutations in human-relevant exons...

  19. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...the more friends they have in common. Only the largest connected part of the follower network is shown...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has...
Showing: 121 - 140 of 402 results