We narrowed to 868 results for: REN

...soils. Dendrobatidae (Lauren O’Connell)  Poison frogs are great for studying parenting strategies and make...land. Current tools are all for Xenopus (aquatic frogs), but there are few genetic tools currently for ...Genomic Tools) Program and I met scientists from 21 different labs who clearly also share this wonder of the...convergent evolution because electric organs arose in different branches of the evolutionary tree at least 6-7...editing in the brain allows the squid to create a different protein. Did you know that most cephalopods die...behavior. For example, two similar species have very different social behavior. One is monogamous and one is ...new tools being developed and shared for many different organisms. Do you have a favorite organism you...

... can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local hydrogen bonds...Prokaryotic chromosomal replication. Adapted from BioRender template. Since DnaA binding is necessary ...proteins also contribute to this regulation in different ways: methylating newly synthesized DNA, blocking...numbers can allow RepA to link iterons from two different plasmids, forming a pair of “handcuffs” that blocks...RepA proteins form complexes between iterons on different plasmids in a process called "handcuffing," which...folding, blocking replication. Created with BioRender.com. Plasmids under stringent control of replication...was updated by Emily P. Bentley in June 2024.  References and resources Further Reading Quiñones-Valles...

...going to cite hundreds of references) and it’s better to keep your references organized from the beginning...the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this post...criteria to shape the document. Get and use a reference management program (e.g., EndNote, Papers, Mendeley...Mendeley, etc.) You’re going to be managing a lot of references. I cite as I write, meaning I use the software...in real time as I wrote, so each statement was referenced even in the roughest version of the draft and...that people can go back and read the original reference for the data. For example, we summarized how frequently...place to get a sense of the similarities and differences among proteins in the class. Offer your perspective...

...educational training, and conferences, and synthesizing this knowledge into a coherent narrative that gives...best with diverse teams, to leverage the preferences of different personality types, and to devise strategies...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...companies. Medical Affairs groups work on building awareness among physicians and scientists about clinical...consulting firms coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs...prepare you for a successful career in several different fields. While some people continue to enjoy the...BioCentury can be an engaging way to learn about the trends and workings of the healthcare industry. Finally...

...doping to forensics, DNA sequencing to drug testing. You can find Nick on LinkedIn. References 1. Skloot...Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification ...the cell lines deposited with them and used by different researchers are free of contamination and match...from this bank. I am often asked, “What is the difference between materials available from commercial repositories...the same cell line from a colleague?”. The main difference is the QC and validation carried out by the commercial... The same test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place... produce a new batch once you’ve passaged your current batch too many times or you suspect there is contamination...

...are communicating across disciplines or doing different tasks. But these challenges can be exaggerated...equal opportunity to contribute. Using video conferencing tools like Zoom or Google Hangouts is a great...great way to include researchers from different locations. Having virtual face-to-face meetings as opposed... collaboration includes scientists working in different time zones, it’s important that everyone can access...project at the International Cancer Immunotherapy Conference in Frankfurt, Germany When funding and circumstances...techniques are being performed the same way at different sites. I did a lab exchange as part of the collaboration...allowed me to investigate my protein of interest in a French clinical cohort, neither of which I would have ...

.... The plasmids within it contain a variety of different promoters followed by leader sequences (encoding... at the BIOFAB showed that expression of many different genes was predictable based solely on the promoters...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...or protease sites to remove the epitope tags. Different plasmids in the kit can be used to purify proteins...prokaryotic expression in recent years, with many different systems now available. However, it can be difficult...ORF clones to allow testing and comparison of different cell-free expression systems; the Cell-free expression...bind specific RNA bases. By swapping out these different modular repeat segments, you can create a PUF ...

...what we need to know in order to even assess the current state of research became more clear throughout ...that, even though postdoc associations across different universities are trying to compile more comprehensive...path,” but individual institutions can have many different names for the positions commonly thought of as...the Starting Salary for Postdocs to $50,000 (the current median postdoc starting salary as reported by Paula...Student Career Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. ...achieved. Of note, it was highlighted during the conference that just increasing funding for academic research... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...

...microscopy and beautiful imaging. References and Resources References  He, Y., Zhang, T., Sun, H. et al...killing the untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a ...desired plants! PCR or fluorescent markers are currently used in these situations to select against CRISPR...such as cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments...it for selecting transformation events and am currently planning to use it to simplify my work with an...(Bottom panels) RUBY expression can vary from different transformation events likely due to insertional...lower expressing RUBY plant is a pale pink in transparent tissues, or in green tissues is slightly darker...

...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney...were isolated from is still a mystery, but it’s currently assumed that the cells came from an aborted fetus...genetically female. HEKs are generally cultured as an adherent monolayer, though derivatives have been adapted...have some neuronal qualities.) While HEKs probably aren’t a great model of kidney cell biology, there are...

...of many complex cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these...genes or to introduce heterologous genes, but the trend of research has shifted in recent years toward tinkering...system for multidirectional control The CRISPR interference (CRISPRi) model consists of a catalytically ... multiplex control via scRNA design is readily apparent in metabolic engineering. The authors elegantly.... cerevisiae by using three RNA programs to differentially alter the levels of five genes that encode ...sequentially rerouted the metabolic flux to generate differently pigmented derivatives of violacein. Using dCas9...that will still recognize guide RNA but with a different dCas9 binding module, alternative sets of transcriptional...

...available jobs, fields, and trends “Never Leave an Empty Seat” at conferences and seminars, as this is ... path to professorship. But recently a downward trend in funding and space in academic research labs has...traditional academic setting and to shape and strengthen interests and skills. Although the word “extracurricular...potential EAs and which professional skill sets are strengthened. This short list highlights the ubiquity of ...particular field of study Assess your interest in a different field Outreach activities (e.g. Volunteer...Marketing Communication Business models Conference or club organizer Management Leadership...know where graduate students and postdocs are currently getting hired? Let us know what topic you'd like...

...seeing adoption of the technology in different labs for different applications.” Li’s team noted that ... genetic diseases, and several treatments are currently being tested in clinical trials. It is speeding...cells of patients with cystic fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome...for selection and optimization. In addition, the current work in developing anti-CRISPR proteins aid the...possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” ...at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294-019-01040...its Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174...

...of the program beneath it. You can jump to the different time stamps in the description to watch any sections...precisely. You can find a great discussion of different types of replication in Nosek and Errington 2017...another’s reagents with the confidence that they aren’t simply doing an experiment wrong. However, even... a plate or running a gel have intricacies that aren’t always mentioned in static written protocols. It...scientific findings. This makes sense on many different levels - it’s hard to process large amounts of...to get a publication (for better or worse, the currency for success in academic research). Initial positive...review our experimental designs and we need to be transparent about the reasons we alter these designs if we...

.... Unfortunately, there really aren’t any effective off-target reference controls. Off-targets and your...they do arise. Happy CRISPR’ing! References and Resources   References Rabinowitz R, Offen D. Single-Base...effects should impact your experimental design differently. Yes, it is always better to reduce off-target...mutation, off-targets must be quantified slightly differently. For a comprehensive approach, RNA-seq would ...acknowledged as a potential limitation in a study. Strength in numbers If you assess your off-target frequency...to control for off targets as well as clonal differences. If you are performing CRISPR library screens... as a transfection or transduction control, a different gRNA would have distinct off-target sites, eliminating...

...imaging for advanced monitoring of different factors, in different or the same cellular location, all ...covered with packaging plasmids! References and Resources References  Bhalla N., Jolly P., et al. Introduction...biosensors provide quantifiable readouts for levels of different chemicals, hormones, proteins, etc., that you ...and zinc. These ion sensors can be targeted to different cellular locations or tissues depending on the...acceptor of a FRET pair on actinin will produce different signals based on changes to the structure of actinin...organisms, but AAVs make it possible. In AAV systems, different serotypes can be used to affect or determine viral...

...isolated from different organisms and engineered Cas9 variants that were able to target different PAM sites...activity of Sniper-Cas9 to different Cas9 variants using gRNAs of different lengths (Lee et al., 2018)...found in other species can recognize a variety of different PAM sequences. Researchers have also used experimental...specificity can target more sites. Created with BioRender.com. Specificity Specificity is the assessment...relative to eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random and directed mutagenesis...choosing a Cas protein for your experiment. References Hu, Johnny H., et al. "Evolved Cas9 variants ...

...skills that were quite different from mine. Learning to bridge those differences was a pivotal moment in...technical problems in the lab, management involves a different skill set: mentoring, setting strategic goals,... day-to-day details. Ignoring individual differencesManaging a team isn’t “one size fits all”. Each team...team member has unique motivations, strengths, and learning styles. For example, during my time as a lab...autonomously. Failing to recognize and adapt to these differences could have led to frustration, micromanagement...cohesive, productive environment. For example, in my current role managing a cross-functional team on a biomarker...team meeting to highlight their complementary strengths. By encouraging alignment on goals and clarifying...

...performance under different imaging conditions (e.g. different emission filters) or different set-ups. Many...uptake by cells. To correct for this variation, a reference fluorescent protein that is expressed at an equal...be used. An assay developed in our lab uses a reference protein that is translated from the same open-...between the fluorescent protein of interest and the reference protein (Goedhart et al, 2011) allows for a precise... a set of fluorescent proteins (with the same reference protein) under identical conditions, a ranking...for co-expressing mTurquoise2 with mVenus as a reference protein is available from Addgene. Another way...

...Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied...Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently... first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused...technique’s versatility and adaptability to many different contexts. Improving Nano-lanterns In 2015, Takai...characterizing Nano-lanterns and eNL for many different applications. For long-term imaging, especially... for excitation, you instead have to rely on differences in emission wavelength. Filtering the emission...

...ganglia in movement disorders.   References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze...GFP) and mCherry, two of the most common tags currently used, are approximately 240 amino acids in size...in monkey neurons, we next hypothesized that a different tag fused to the hM4Di might improve the transport...Surprisingly, we found that this small change made a big difference. Replacing the mCherry with the HA tag resulted...Overview of Affinity Tags for Protein Purification. Current Protocols in Protein Science 73: . https://doi....doi.org/10.1002/0471140864.ps0909s73 Additional references on the Addgene blog Browse Fluorescent 101 blog...Find more about the many types of protein tags References on Addgene.org Check out Addgene's Chemogenetics...

...any end goal not available with current techniques like PGD.  Reference Ma, Hong, et al. "Correction of...al. designed plasmids containing Cas9 and two different gRNAs, and electroporated them with an ssODN repair...editing through the NHEJ pathway. Created with BioRender.com. In later characterization studies, the ...Moreover, introducing new DNA seems impossible with current tools, thus quieting some fears about the creation...creation of “designer babies.” The differences in editing between iPS cells and embryos make a case for conducting...of such a treatment on top of the expense of IVF render this argument less convincing at present.   Another...embryos to only 60%? Unfortunately, given the differences between CRISPR repair in embryos and iPS models...

...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...arise during treatment or are preexisting in the parent population prior to the start of treatment. They...They hypothesized that, if they labeled the parent cell population using the library, propagated it, separated...labels - i.e. they would always come from the same parent cells that represent the preexisting resistant ... determine using NGS because the error rate of current NGS approaches allows for a detection limit of ...up-front quickly. This approach is in contrast to the current treatment paradigm where resistant populations ...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

...in with the current literature. Finally, if you give your talk or poster to enough different kinds of audiences... NIH, in the United States) Strong letters of reference that attest to your talent and expertise as a ...data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly thinking...Leader position applications require 3 letters of reference- the graduate school mentor, the postdoctoral ... and your research well. Who could fill these different roles? A second or third mentor. Therefore, seek...the job market. Additional mentors are not so different from having a thesis committee in graduate school...presentations and will be able to provide letters of reference, informal recommendations, more positive grant...

... use of several Cre transgenic mice targeting different brain areas enabled the drawing of a detailed ...the genOway press release for more information. References Abdallah, Khaled, et al. "Adeno-associated virus... delivery of Cre recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed ...for analyzing the neural circuits of behavior." Trends in cognitive sciences 15.12 (2011): 592-600. PubMed...Optogenetic approaches to study the mammalian brain." Current opinion in structural biology 57 (2019): 157-163...and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991): 489...conditional gene modification technology in the mouse." Current protocols in immunology 105.1 (2014): 10-34. PubMed...

...My name is Lauren Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst...concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind our "Meet an Addgenie...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...can have flexibility and be up to date with the current world. Bioinformatics it is.’” Though she misses...Shannon Rinaldi values loving what she does. Her parents told her that if she found something she loves ...-op. It has been so insightful to learn about different career paths, successes and failures, values, ... Lauren Flynn...

...examine three things: Aligning the NGS result to a reference sequence to confirm backbone elements.   Confirming...deposited plasmids is to align the NGS result to a reference sequence like a known backbone sequence. We’re...through BLAST or by direct alignment to an NCBI reference sequence or a sequence provided by the depositor...synthesized regions that have no publicly available reference sequence or plasmids containing genes with many...the depositing laboratory confirms that these differences are expected and do not affect plasmid function...challenging to QC by NGS as they are often quite different from one library to another. They also differ ... Repository sometime soon. Stay posted!     References Guerin K, Rego M, Bourges D, Ersing I, Haery ...

...antibody for your application!  References and resources References Lu, Q. L., & Partridge, T. A. (1998... while polyclonal antibodies contain a mix of different isotypes and subclasses. They each have their ...of clones and thus the potential to bind many different sites on the target protein they tend to have ...polyclonal primary antibody contains a mix of different isotypes and subclasses, use a broadly reactive...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide... be worth considering an antibody raised in a different species.      Table 1: Isotype considerations...targets, look for primary antibodies raised in different, and distantly related, species, and secondary...

...collaboration. The field already has comprehensive reference pages and curated databases for scientists including...discussion on the best strategies and protocols for different experiments.  In this blog post let's take a ...atlas of every C.elegans  neuron. Scientists are currently doing this using two approaches: single-cell RNA-sequencing... peer-reviewed and assigned a DOI that can be referenced. In addition the data published in microPublications...embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor the effects of RNAi...RNAi using four-dimensional contrast interference (DIC) microscopy and computer imaging processing. The... back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN Project:...

...library protocols you can use as a reference. Table 1: Examples of Different Pooled Libraries at Addgene.  ... plasmids in your library (Are the inserts of different sizes?), and the desired coverage for the library...library amplification protocol. Created with BioRender.com.   The most important factor to consider...For this reason, if your library has inserts of different sizes (such as a transcription factor overexpression...your growth time (e.g., 16-18 hours). Finding reference protocols There are many decisions to make when...pDNA Library Amplification protocol are useful references. Skim through our Pooled Library page to find...“one-size-fits-all” protocol! Each library is different, and you should always experimentally test the...