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We narrowed to 435 results for: Trac

Showing: 181 - 210 of 435 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  3. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...PubMed user searches, such as clicks to read the abstract or full text of the paper, publication date, a...publications and figures using machine learning to extract important features such as influential citations...

  5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Pooled libraries are a bit more challenging to...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...replaces the targeted gene and provides a way to track successfully engineered cells. Similar to transgenic... the cell can occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to...

  7. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...can be accomplished with an exciting title and attractive figures. Next, the poster should be legible from...presenting. The latter is catchy, but beware. A distracting background or clashing colors can take away from...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...and requires only one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

  10. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... produce low levels of virus and can be used to track carryover. Functional titer Functional titer is ...Learn How Lentivirus Has Been Used for Lineage Tracing Additional Resources on Addgene.org Learn How...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the world. With...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...our ideas got pretty good. We started doing extracurricular research on some of them to see how feasible...SynBio Axlr8r We limped along with very little traction for quite a while, and even had some serious self-confidence...

  13. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the ...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The mythical... committees as a tenured professor or signing contracts when working for a business development team, ...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent 0.001%...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair from...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program...

  21. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...1993). But why did this technique fail to gain traction? It is likely that both vector amplification with...performing multiple modifications will require ultracompetent (109 CFU/μg) cells. Primer design Using specific...

  22. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... and regulations impacting grant management, we track grant opportunity deadlines, and we ensure that ...questions when you’re negotiating a new job or a new contract. Connect with other grant professionals on LinkedIn...

  25. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...vector, incubated for 72 hours, and genomic DNA is extracted. Primers and probes targeting integrated copies...

  26. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing...intraperitoneal doxycycline injections to enable their tracking with ultrasound imaging (Fig. 2d). Creating a ...

  27. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... to tackle it. It’s easy to get distracted by an email or sidetracked by a colleague. To innovate, you...

  28. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ... the transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...was successful at all. Once we were on the right track, we switched to luciferases to more easily quantify...

  29. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...with AAV sgRNA delivery make the SPH mouse an attractive tool to probe gene-function relationships in ...cell populations makes Cre-dependent SPH mice an attractive alternative. Whatever your application, first...

  30. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation promotes...
Showing: 181 - 210 of 435 results