... like Cas9. The Cascade complex doesn’t usually carry out the function of the specific CRISPR type but...is not known, but it’s suspected that they don’t carry out the same types of adaptive immune functions ...can hijack machinery from other CRISPR systems to carry out various functions. Class 2 Class 2 CRISPR systems....2021.671522 Yan, W. X., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity...

... you can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged...That way, you know you’re getting the serotype and cargo you requested at the quality you expect. Similar...serotype, place your order when ready, and we’ll take care of the rest.    We think Packaged on Request AAVs...

...prepared Tom for an academic career. Career advice for anyone pursuing a career in academia. Learning how...

...first to be infected in the United States while caring for a patient, despite wearing protective gear,...gear, and now comes news of a second Texas healthcare worker with symptoms. The World Health Organization... in EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York...

...chemogenetics, plasmids, fluorescent proteins, careers, and more. These resources can now be found in ...Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela...fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific...

...Resources Science Careers: Improving your work-life balance Balancing the Scale: NSF's Career Life-Balance ...anyone leaving the lab receives send-off via a gift, card, and trip to the pub. Dr. Ellis adds “we probably...

...such as the NIH BEST award) and availability of career tools (such as the National Postdoc Association...Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav, Associate Professor of Systems ... to take advantage of all the possible science careers. She advocated for increasing the value and priority...

... to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes a good manager? If that is all it ...is not the same as not being a bad manager (see cartoon above – what type of bad manager have you had?)...best managers have learned to get results with the carrot, and not the stick.     This is the first in a...

...ability to tell stories a similarly crucial skill for career advancement. Scientists do and should tell stories...can be learned, and will serve you well in your career. Story Telling at work Don’t believe me? Here’s...communication Find interviews scientists in SciComm careers Check out our series on transferable skills ...

...by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called SOX10. To ...technology to create cell lines and/or mouse models carrying different SOX10 mutations. More specifically, ...kids in Thailand will receive much better health care in the future based on our studies.” Thank you to...

...confidence to launch new and diverse careers by taking part in career panels, and volunteering for scientific... “The Salmon Leap for PhDs: Swimming upstream a career from academia to industry”. To date, aside from...

...our excellent service and accuracy involves caring and careful Addgenies, but also a lot of good tracking... Bryan Roth’s lab from the University of North Carolina, Connie Cepko’s lab from Harvard Medical School...

... it, virtually every milestone in a scientist's career is mediated by some kind of communication to another...communication will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news...Additional resources on the Addgene Blog Learn about careers in science communication The strength of story ...

...Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate...pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry...

...can then build on this research and effectively carry out their mission of creating new forums and opportunities...were generated by large agricultural companies and carry associated intellectual property concerns – patents...to construct our color changing flower we are carefully making our way through the IP landscape, using...

...that my research will have a direct impact on healthcare and the community. As a result, I have occasionally...target and light-up bacteria unwanted in food, healthcare settings, etc. What project in the Collins ...co-founder of a biotech startup, Mike explained, his career transitioned away from the bench and became all...

...makes them that way. Is there a senior person whose career path you are interested in following? Someone you...notify your network that you’re on the hunt for career advice. Your family and friends know you well and...groups will often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent...

...anyone who is planning or might need to plan for a career outside academia (that would be pretty much all...managers will think it is odd. For those planning a career in academia, it may not be required, but many academic...You can use LinkedIn before an interview, but be careful If you are lucky enough to have a schedule before...

...While the antibodies present throughout our bodies carry out plenty of roles just the way they are, the research...lysines, sulfhydryl groups from cysteines, or carbohydrates from glycosylation (Dennler, et al., 2015). ...bonds between antibody chains. 3) Endogenous carbohydrates added to antibodies post-translationally. 4)...

...confidential process among a few individuals.  Early-career researchers especially enjoy the ability to post...Puebla I (2022) A guide to preprinting for early-career researchers. Biol Open. https://doi.org/10.1242...Hensel Z (2019) On the value of preprints: An early career researcher perspective. PLoS Biol. https://doi....

...recombination scar encoding eight amino acids, but Golden Gate assembly can be designed to be scarless. Golden... in the final construct, allowing for precise, scarless cloning. The cloning scheme is as follows: the...

...Conveniently, plasmids carrying genes for bacterial toxins like colicin typically also carry the necessary resistance...century! Nevertheless, the transfer of plasmids carrying these genes between bacteria can also have serious...

...ultimate goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020...JC, Shon DJ, Fox DM, Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (...Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine CL, Zuo T, Lin J, Zuiani A, Feldman...

...which means it requires multiple Cas effectors to carry out genomic editing. Specifically, these types of... using a gRNA and can then recruit Cas3, which carries out the brunt of the mechanical work for editing...The reeling mechanism The reeling mechanism is carried out much like the name implies — after nicking,...

... Andrew and Maria were skeptical. The team was careful to test the possibility that this might just be...proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved...

...figure at right for an example). Look out for carbohydrates Certain strains, including HB101 and derivatives...derivatives, release large amounts of carbohydrates that can inhibit the cell lysis process. To ensure that..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

...for viral genome sequencing (VGS). Why should you care about viral quality control? AAV vectors are produced... sequence. The resulting list of hits is then carefully reviewed. As mentioned earlier, it is common to...the contamination can be identified, and we will discard any AAV prep we suspect is contaminated. For AAV...

...advantage over Taqman is price point, which should be carefully balanced against the limitations of the assay....left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains a primer and probe set for...Cloning Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number Variation with Droplet...

...Transformed cell lines Transformed cell lines Cargo Nucleic acids or protein Nucleic acids Nucleic acids...Microinjection Cells Many types Single cells Cargo Nucleic acids or protein Nucleic acids or protein... nondividing Dividing Dividing or nondividing Cargo Nucleic acids Nucleic acids Nucleic acids Throughput...

...spread even when costly. If even a single organism carrying a synthetic gene drive were to escape the laboratory...trust in scientists to develop new technologies with care and humility. Nothing would damage that trust more...aaa5945 (2015). doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M....