We narrowed to 820 results for: APP

...to take, and if something does happen, ensure you can receive appropriate treatment and/or monitoring. ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required and...start an experiment, just in case something does happen.   Finally, the best way to be safe is to know ...valuable tools without endangering yourself or others. Happy transducing! Resources Resources on Addgene.org...

..., its dimeric nature can interfere with some applications. Several labs have been working to produce monomeric... for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https://doi.org/10.1038...successful in primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants...proven itself a good option for essentially all applications. Addgene’s plasmid collection includes over ...clone (M2) has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here...

... editing in primary human cells  Cell therapy approaches can use base editors to correct mutations in ... reduced off-target editing frequencies. For applications that require high DNA editing specificity, for... for example for potential therapeutic approaches, the authors recommend mRNA delivery, the V106W version...deamination, an important attribute for therapeutic application. ABE8s demonstrate an overall improved base editing...editors with increased activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038...

.... We both had an applied science bent, so the engineering aspect of this job appealed to us. However, ...possibilities in life for employment?  It never happened – there was never a “moment”. But there were a...about continuing with the business. We ended up applying to a business accelerator freely stating that ...your thoughts with us in the comments below. We’re happy to answer questions about this transition (so far...

...carefully) holding plates up to the light, where they appear as opaque dots (colonies should contain at least...only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct temperature...and not spread out onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the...densities, then dispose of the plates that do not appear to be yielding well-spaced clones. For rapidly ...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

...contain rAAV. To minimize contamination, use appropriate decontamination procedures (Korte et al., 2021...surfaces that come into contact with the rAAV and apply common cell culture practices diligently throughout... toxic transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your...al., 2010). For certain in vitro and in vivo applications, you can consider using unpurified AAV from ..., 2017). You can learn more about common AAV applications by reading our Viral Vectors 101 series!  This...

...uncontrolled cellular proliferation. We have appreciated for some time the causes of this uncontrolled...cellular machinery necessary for division. This approach also kills dividing non-cancerous cells, such ... University of California, San Francisco, is approaching the Ras problem by exploiting the different mutant...genes from the Ras signaling pathway. Its major appeal is that it enables the simple construction of vectors...any of these genes through several different applications such as fluorescent protein fusion studies and...

...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few...tips. Based on the discussions in the forums, it appeared that many researchers were having difficulty achieving... target site. Overall, 64% of researchers were unhappy with the new method and 65% wanted to wait until... the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute Plasmids... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

...in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first gene therapy for the treatment...AAV-based gene therapy treatment for a genetic disease approved by the FDA is for a rare eye disease and not another...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...medicine. References and further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

...lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...that would compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers...opinion, it was more important to communicate the applications of CRISPR over the details of how it worked....analogy that involves student participation, make it happen! How should we use CRISPR? How shouldn’t we use... I came out of the experience with a renewed appreciation of our science teachers’ difficult-but-rewarding...

...with an abundance of protocols and examples of applications from across disciplines now available. In this...Tissue expansion is not compatible with in vivo applications and there’s just not much to be done about that...and Stiles, 2022). Fourth, signal continuity can appear reduced in ExM, which could impact how you interpret...pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should...especially when paired with complementary experimental approaches. Since it arrived on the scene, ExM has helped...

...variations in gene expression, they found that for approximately 40% of the identified genes, the genes were ...lines from individuals of African descent have approximately 20% more single nucleotide polymorphisms (SNPs...ancestry-associated cancer dependencies are undercounted by approximately 20%. In some cases, this may lead researchers...map. Now, any data that came from CRISPR guides mapped to a SNP would be ignored. Working with Lia Petronio...8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, ...

...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The... after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period of 30 minutes...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...recap of the pros and cons of each antibiotic and happy selecting!   Antibiotic Resistance Stability...

...move from lab to office will likely happen eventually. If you approach that transition dreading the change... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...thinking” in the position to which she was applying. I probably did not respond to her question as well then...adjustment period will be that much longer. If you approach the transition embracing the opportunity for growth...

...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop... tools we make available. This prioritization happens for good reasons. We began offering our viral vector...remains the more requested option, and jGCaMP8 appears to be growing in requests faster than jGCaMP7 (...viral vectors. This process builds on our former approach and involves reading primary literature, soliciting...redesigned Data Hub in 2022. It’s influenced the way we approach our viral vector educational resources, from eBooks...

... tangible and applicable to your situation.   Thinking Outside the Box: Team Approaches At some point ...required to develop new projects and test novel approaches, a necessary skill set for any scientist. In ...lab   Thinking Outside the Box: Individual Approaches Whether it’s finding the source of contamination...creative skills you have developed in the lab can be applied anywhere! References and Resources References ...

...to generate new pEcgRNA. pEcCas/pEcgRNA can be applied not only in coli K-12 strains, but also in E. coli...genome editing in microorganisms which could be applied to optimization of genome editing tools.   Jieze...engineered cells for green chemistry and biomedical applications.   Yiwen Zhang entered the Sheng Yang Lab of...research focuses mainly on the development and application of a multiplex genome editing system based on...Escherichia coli Genome via the CRISPR-Cas9 System. Appl Environ Microbiol 81:2506–2514 . https://doi.org...

...In this Plasmids 101 post, we’ll cover why this happens, how it might affect your work, and how understanding...rely on chance (or random diffusion) to make this happen.  There are several different versions of the plasmid...way to bacterial cloning glory, scientists have applied these concepts to deal with the more sinister plasmids...pestis using plasmid incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111...Bacterial plasmid partition machinery: a minimalist approach to survival. Current Opinion in Structural Biology...

...connection you made at the job fair when you formally apply for the position. This post on Business Insider ...re likely to find current job openings. A good approach is to do this first for companies at which you...connection. That way, once you’ve identified an appropriate job opening, you can send your contact an email...the assistance of recruiters for identifying appropriate job candidates. If you do receive such a call...those relationships. Then when you are ready to apply for your dream job, you’ll be in the best possible...

...contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp...website invites you to input the target region (approximately 500bp is best) and select the organism. It will...different types of gRNA design software. When you are happy with your selection, ordering the sgRNA is simple...process, you should first develop primers to the approximately 500 base pair sequence surrounding your gRNA...

...exist in the real world and corrective approaches and appropriate controls are required to make this method... this technique has revolutionised the way we apprehend molecular complexes and is still a very useful...experiment. You can then choose the most efficient approach for your own system. The simplest and the most...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including... feng, Liu, N. hua, Shu, L. yuan, & Sun, H. “Application of avidin-biotin technology to improve cell adhesion...

...one of two ways - each junction resolution could happen on the crossing strand (horizontally at the purple...strand. While this pathway may not be immediately applicable in DSB-induced genome engineering, it has biological...that can be harnessed by researchers for many applications. Your favorite Cas enzyme, a guide RNA to your...Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014)... PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS...

...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower... in the fluorescent properties, pYtags are an appealing tool for monitoring RTK signaling.  Figure 4:...lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán et al. 2022...screening platform developed here is also a promising approach for developing sensors for other targets. Figure...

...sell them. Types of Conflict Before explaining my approach to conflict resolution, it’s important to recognize...day-to-day operations and keeping our customers happy, but inevitably there will be gray areas that either...you can help everyone walk away from a conflict happy, feeling they each got enough of what they wanted...Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based on various levels...yourself gravitating most frequently towards these approaches, you may need to put more conscious effort into...

...decades. Widely used across various research applications, they have become indispensable tools for manipulating...-lasting changes in cells. However, for some applications, integration might be considered a double-edged... certain types of clinical or basic research applications. Considerations for IDLV use If you’re reading...Integrase-defective lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org...lentiviral vector: prospects for safe clinical applications. PeerJ, 10, e13704. https://doi.org/10.7717/...

... talks and how to make them serve you well. The happy byproduct might just be a career opportunity. The...non-academic settings. Many of these guidelines will apply to any type of science presentation, but, for more...and venue before tailoring each presentation appropriately.  Find out who will be listening.  Are they ...goal is to get the listeners to be thinking “What happens next?”  Describe how you pushed to a solution or... use no notes and can concentrate on delivery, rapport with the audience, and their own excitement about...

...ways to clone DNA fragments which have provided appealing alternatives to restriction enzyme cloning. These... amplification with primers that contain the appropriate homologous sequences. NEB recommends an overlap...sequences remaining in the final product. The long overlapping region between fragments also better ensures ... of the fragments as compared to the smaller overlapping sequences left with a restriction digest. A study...cloning kits available which are all based on long-overlapping regions, have no requirements for restriction...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place....org with any thoughts, questions, or feedback. Happy reading! ...

...it got “dirty”, with multiple sequence traces overlapping each other! When the count was done, 75% of the...but not least, when using CRISPR, remember to appreciate the fact that it was first discovered in an obscure... with Biogen and is particularly interested in applying CRISPR for mouse genome editing.     Haoyi Wang...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-...

...from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More #agarart from our lab @SILS_UvA...display @Micropia! #SciArt #MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega...recycled lab materials into holiday decorations. We appreciated the creative uses of old posters, pipette tip...to New York City. Check out their video below. Happy Holidays from the @SikoraLab! This year, @BordeauxFoSho...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...detection, treatment, and management. We are incredibly happy to hear that Gladys will be using plasmids in a ...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

...blog post will discuss differences between these approaches, and provide updates on how best to design gRNAs...capture the mRNA cap, provides the most accurate mapping (Radzisheuskaya et al., 2016). In this case, location...location and sequence are of approximately equal importance in design – an optimized sequence will do ... using knockout or transcriptional modulation approaches. Avoiding off-target effects The off-target ...fully predict which sites will and will not show appreciable levels of modification. Whole-genome sequencing...

...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

... recent discussions of this apparently worsening crisis recently appeared in eLife: "A Fair Deal for PhD...news and updates. Scientists must do science to be happy. What do we have to change to ensure that all scientists..., there are some easy ways to get started. I am happy to work with any group of trainees interested in...

... is supposed to be impartial. The best science happens when scientists have 1) the courage to make mistakes...degree. It was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying...tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how to engage with friends...galilei-dialogues-concerning-two-new-sciences). This is the approach policy makers take in abusing climate science ...defund science and stay silent. I was overwhelmingly happy to see so many others come out who feel the same...

...through an electric field to deflect them into appropriate containers. The machine is useful for distinguishing...on in vitro studies where it was difficult to apprehend the key role of specialized cell types and organs...Good two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins...this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely tune the...creation of mouse lines that can be used in fate mapping experiments. Immune response dynamics are much ...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept...

... a serious academic subject, it is now being appreciated on an aesthetic level as well. Beautiful microscope...can be used as a way to better understand and appreciate the organism, for both scientist and non-scientist...the difference in some areas of conservation.” Appreciating science as an art form taps into an emotional...matter is seen to have increased value and is appreciated by a wider audience for more than just its biological...

...for both transient and stable gene expression applications in plants. This smaller, easily customizable... susceptible to Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...only for plant engineering and biotechnology applications, but also for foundational studies of promoter...