960 results

Get advice on tagging optogenetics and chemogenetics receptors to ensure that your receptors express and/or localize to the membrane.

May 11, 2021, 1:15 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

Sometimes, it's hard to turn your research into a paper or a grant. Here’s a list of tools and resources that might help make academic writing easier.

April 28, 2021, 1:15 p.m.

Get free science-themed Zoom backgrounds from Addgene! Backgrounds include plasmids, viruses, DNA, and more.

April 22, 2021, 1:15 p.m.

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

April 20, 2021, 2:30 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Addgene has reached 100,000 total reagents in the repository! The next milestone coming up is 100,000 and we'd like you to guess the date that happens (a contest).

April 8, 2021, 7:25 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Play along in the scavenger hunt to find Addgene pets all over the blog. Enter to win Addgene swag!

April 1, 2021, 1:15 p.m.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

March 23, 2021, 1:15 p.m.

Addgene welcomes Julie Cicalese, former Chief Human Resource Officer for Harvard Medical School, to the Board of Directors.

March 17, 2021, 2:40 p.m.

An overview of the western blotting process from separating proteins by size, transfer, staining, and visualizing.

March 8, 2021, 2:41 p.m.

Master the streak plate technique using a mixture of E. coli strains expressing a different chromoprotein.

March 2, 2021, 2:15 p.m.

Learn about the Professional Development Hub's work to support science trainee career transitions. Register for the event covering findings from their report!

Feb. 25, 2021, 2:15 p.m.

Trying to decided between a master's or a PhD? Here's five questions to ask yourself.

Feb. 16, 2021, 2:15 p.m.

GPCRs are receptors that respond to stimuli and elicit cellular responses. Learn about the parts of GPCRs, how they work, and tools to study them.

Feb. 11, 2021, 2:15 p.m.

Find tips for hosting a great virtual lab meeting with ideas for online collaboration and space for brainstorming and discussion.

Feb. 9, 2021, 2:15 p.m.

If you're looking for a STEAM internship, find tips for a successful application season in this blog post written by the founders of STEAMid.

Feb. 2, 2021, 2:15 p.m.

Use this quick reference sheet for a quick refresher on the basic facts about CRISPR and genome editing.

Jan. 27, 2021, 2 p.m.

Welcome to the Addgene blog! Here you'll find all the info you need on plasmids, CRISPR, viral vectors, fluorescent proteins, science careers, and more.

Jan. 25, 2021, 5:54 p.m.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Jan. 19, 2021, 2:15 p.m.

See the winners from Addgene's 2020 Deck The Lab contest! Contestants could either decorate the lab or make a science-themed winter decoration from home.

Jan. 14, 2021, 1:30 p.m.

Just met some great people while networking? Check out this article for tips to maintain connections and tips for what not to do.

Jan. 12, 2021, 2:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

Find out what were Addgene's most popular blog posts in 2020!

Dec. 29, 2020, 2:15 p.m.

Enjoy 12 new CRISPR articles from Addgene's 12 Days of CRISPR!

Dec. 22, 2020, 2:15 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

An open-source biosensor platform, TRUPATH, measures the association of G protein signal transducers using bioluminescence resonance energy transfer.

Dec. 14, 2020, 2:15 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

Learn about the parts of the AAV RepCap helper plasmid and how they help to package AAV.

Dec. 3, 2020, 2:15 p.m.

Wondering what a software product manger does? Check out this article to see a day in the life of a software product manager in the life sciences.

Nov. 24, 2020, 2:15 p.m.

Discover the history of Rett Syndrome research, the role of the MECP2 gene, and some of the latest breakthrough therapeutic approaches for Rett Syndrome.

Nov. 19, 2020, 2:15 p.m.

Join Addgene for Depositor Week from November 16 to 20, 2020 as we celebrate the role of Addgene depositors in open science!

Nov. 16, 2020, 2:15 p.m.

Should you codon optimize your gene or not? Here, we discuss several reasons for and against codon optimization.

Nov. 12, 2020, 2:15 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

New channelrhodropsins, called ChRgers, avoid intracranial injections and fiber-optic cable implantation for in vivo optogenetics experiments.

Nov. 3, 2020, 2:15 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Find six out-of-the-box ideas for professional development in this virtual world.

Oct. 20, 2020, 1:15 p.m.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Oct. 13, 2020, 1:15 p.m.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Oct. 8, 2020, 1:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Sept. 29, 2020, 1:15 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

Sept. 22, 2020, 1:15 p.m.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

Sept. 17, 2020, 1:15 p.m.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.