Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 610 results for: STO;

Showing: 301 - 350 of 610 results

  2. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...calcium events), Greater brightness, Less signal distortion from photoswitching or photobleaching Improved...addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged on Request ...

  3. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ... tasksDelegation can feel unnatural for those accustomed to hands-on work. The temptation to "do it yourself...Matteo Tardelli authored the book "Beyond Academia: Stories and Strategies for PhDs Making the Leap to Industry...

  4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope ...

  5. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...space offers STEM programming, workshops, and customized programs to meet the needs of students and teachers...blogger, Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this can be found on the...BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using...

  7. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...box" provided by the manufacturers and enables customized experiments. Projects such as the OpenFlexure...Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed open standard for microscopic...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...the frozen cell line becomes non-recoverable, the stock of the mAb becomes limited, which can be a threat...able to isolate RNA from non-recoverable hybridoma stocks, without having to grow up the cells. This RNA ...

  11. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance...would like to thank all of our depositors and requestors for making their reagents available and for supporting...

  12. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below!   Additional resources ...

  13. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...resources that answer questions our depositors and customers are asking. So how can you get involved? Keep ...

  14. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...involved in central anthocyanin metabolism are well understood. Additional work has also identified and characterized...how you were able to overcome them. Share your stories in the comments below! And if you have developed...

  16. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...technique for monoclonal antibodies.  J Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org...

  17. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time and cost ...any of the numbers in this post is a mathematical story well beyond my ability to tell. But even from these...

  18. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...

  22. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that...the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...

  24. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...2019 Society for Developmental Biology meeting in Boston. The atmosphere was electric! After attending this...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...

  25. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will...

  27. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

  28. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific...

  29. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains...

  30. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid...

  31. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog...

  32. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ...

  33. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for  observation in live cells....

  34. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal...

  35. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific...

  36. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want...

  37. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA...

  38. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...genetics and anatomy, which are not yet fully understood. We attempt to deconstruct and understand this... into it! If you’re in the Victoria area, please stop by. And if not, I suspect you can find a makerspace...

  39. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ... 5) Listen to your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor...Look at the problem from their viewpoint, and brainstorm about solutions that will meet both of your needs...

  40. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...purification, and titration. Can't wait? Browse our In-Stock AAV inventory or use our Packaged on Request service...

  41. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option...

  42. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that...Plasmids for Mammalian Systems Learn about CRISPR History ...

  43. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... cleaving of the target strand is not as well understood but likely occurs in similar regions. This combination...up to hundreds or even thousands of base pairs, stopping either at boundaries defined by anti-CRISPR proteins...

  44. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...particles you need are not available, or the cost of custom viruses are too high for your budget. In those ...round provides the added benefit of producing a stock P1 virus, so that if you ever need to remake the...

  45. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...resulted from our work with teachers, and to create custom models of proteins for researchers. Modeling CRISPR...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  47. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause at the... Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann...

  48. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...levels. Chromoproteins in nature Compared to the history of fluorescent proteins in molecular biology research...research, chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored...

  49. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq DNA polymerase... low during the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction...

  50. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...to tailor your presentation so that it can be understood by your audience. Use your poster as an aid: ... practicing with your colleagues, ask them to brainstorm potential questions you may receive. People often...
Showing: 301 - 350 of 610 results