...Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of...leaving virulent components behind. Minigenomes are stripped down viral genomes containing only the sequences...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...

...tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano Heredia, Peru Patricia Sheen, Professor ...Biology Resources Learn about Global Plasmid Distribution ...

... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...identification of non-dominant ITR sequences.  Restriction digests with the enzymes SmaI and SrfI can also...deletions and point mutations will go undetected by restriction digest methods.    Resources and references...

...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this work..., computing performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com...

... research community. At Addgene, we’ve helped distribute many of these technologies in plasmid form and...libraries. Please note, the pooled libraries we distribute in lentivirus format only deliver gRNA and therefore...like to thank all those who are allowing us to distribute their CRISPR tools as ready-to-use virus and,...

...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

...,850 SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster...citation to the plasmid page on our website. Contributing your results helps other researchers using these...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

... adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in the evolution...which results in less overall dilution and less stringent phage selection. Figure 2: Phage-assisted...

...your antibody.  At some point in your long and illustrious lab career, you will likely accidentally leave...Brightness Chart. Boston University Flow Core. Retrieved January 31, 2022, from https://www.bu.edu/flow-cytometry...overlap. Syracuse University Flow Core Facility. Retrieved January 31, 2022, from https://flowcore.syr.edu...

...This blog post was contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders...cannot offer. Furthermore, companies across most industries tend to hire their former interns to fill entry-level... of internships as a result of the necessary restrictions on close personal contacts. However, that should...

...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...explanation of the assay is depicted in figure 1. The strict correlation between the fluorescent protein of ...

... This post was contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...works. But, those darn cancers have revealed many tricks up their sleeves and most patients still don’t ...or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary conservation...

...These viruses have been distributed in over 200 packages to more than 20 countries. With this initial success...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

... a biotech startup company? Would you like to contribute advice on how to effectively raise money and/... Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and...the Biotech Business - Posted on Nature.com, contributed by Julia Phillips and Fiona Beckman Acknowledgments...

...This post was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "...interleukin-6 (Witkiewicz et al., 2009) which contribute to the development and spread of cancer cells... scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism discovered...

...real-time inside a mouse’s brain.  But the brain is a tricky organ to access, which often forces scientists ...electrophysiology, a technique that’s used to measure electric current of ion channels in living cells. Multiple...-stimulate” their VTA dopaminergic neurons by triggering a burst of light that could activate ChRger2 ...

...plasmid, excluding any restriction enzymes or vector purchases (Table 1). Restriction enzymes and vector ...work done previously, borrow small aliquots of restriction enzymes for one-off projects, or even save yourself...

...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this...especially in the case of older mothers, for whom egg retrieval often results in fewer eggs. However, the potentially...

... held from 22-24 October. This blog has been contributed by guest blogger and Future of Research Symposium...the academic labour market. Her many notable contributions in this area include publishing the book ‘How...How Economics Shapes Science’ and contributing to the NIH’s Biomedical Research Workforce Working Group...

...This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends ...organs, which then return information such as nutritional status. Within the animal kingdom, this information...300 different coactivator proteins individually contribute to cell regulation in a coherent manner. Because...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...Education Project (pgEd), where I currently work, strives to broaden the conversations about genetics and...implications of genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic...

...Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular cell types...types, pairs of synthetic promoter can be used to restrict expression to a single cell type based on the ...cell-type specific promoters could be an easier way to restrict expression of a gene interest in an animal model...

...Scholar library. You can add hashtags to library entries, which can help you organize and search for papers...number of times a paper is shared online, or its altmetric score, is also displayed in your feed. By clicking...us know if you if you have any favorite tips and tricks for navigating the always growing sea of scientific...

...recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing if you can...Cloning specifics Fragmid uses Bbsl, a Type II restriction enzyme that cleaves outside of its recognition..., 93% had the correct banding pattern in the restriction enzyme digest. They sent 82 of the correctly ...

...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...used to reveal brain circuitry, and continue to contribute answers to the complex questions of how the brain...

...Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

...Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the Allen Institute...regions of high homology to the gene with the cut, we trick the cell into using the introduced donor plasmid...Allen Institute for Cell Science. This post was contributed by the gene editing team at the Allen Institute...

...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ...findings will be worth knowing? This can sometimes be tricky, as most of our research builds on ideas and findings... realization that, if we want people to keep contributing to science, it would be helpful for them to ...

...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named...with a FLAG tag (!), but did you know that we distribute ready-to-use recombinant antibodies as well? ...anti-FLAG antibody, both of which are also available in trial sizes. Addgene recommends these recombinant antibodies...

...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...with very little money. So when did inspiration strike us so hard that we upended our lives and took this...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...

...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction...many genomic locations. In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; ...

...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....their germ cells (sets of genomic material are contributed by mother and father). While some mutations can...start working with live animals yet, Harvard’s DRSC/TRiP- Functional Genomics Resources website has in vitro...

...creating the Expression Systems table. Marcy Patrick contributed to the writing of this post. Additional Resources...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...

...default scoring matrix BLOSUM62, but you may want to check the description of the other matrices to see if ...laboratories that deposit their plasmids with us or other entries in NCBI’s numerous databases. As our repository...

...sgRNA-level metrics to assess library performance in viability screens. Based on these metrics the Brunello... CRISPRi libraries with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant...

...and relatively easy? Have all of these things contributed to the steadily increasing distrust the general...that “Addgene, with its easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been ...to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino...

...production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing... D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis is currently ...

...therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector. CD4+ T-cells from patients... lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic...monitoring and follow-up in lentiviral clinical trials. McGarrity GJ, Hoyah G, Winemiller A, Andre K, ...

...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...you’re screening for positive clones! Tips and tricks from the bench Don't pick too large of a colony...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

...competitions (ranging from cook-offs, to corn toss to trivia, and everything in between). Eleven years later...everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve done annually...are the sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something...

...specific DNA sequences. Thus, we've made some contributions in technology development in biology and hope...in these professional activities because I can contribute by, for example, helping to improve the papers...optimized for living for Japanese, which is not trivial, and the grant situation is currently much better...

... mYFP S65G; V68L; Q69K; S72A; T203Y; A206K Citrine S65G; V68L; Q69K; S72A; T203Y ECFP F64L; S65T...Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article. ... Marcy Patrick...

...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State...strand, dramatically reducing the likelihood of detrimental indel formation. After binding with DNA, the ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...

... binding signal for Cas9 and this sequence is a strict requirement for Cas9-mediated DNA cleavage. The...below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells... fluorescent proteins, they found that adding a triple nuclear localization signal or including fusions...CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward loop. ...

...This post was contributed by Joe James from Binning Singletons. The sheer scale of a large conference...their meetings. Amanda Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with...ways. Scientific societies and conferences should strive to provide support to early career scientists, ...

...Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle and Alex Chavez...activation can be made inducible upon environmental triggers. This is done by creating a split dCas9 protein...activation are also reversible: once the environmental trigger leaves, gene expression is no longer activated....

...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...success. Or the client might need help with the intricate task of designing a strategic vision for a portfolio...styles, team situations, and personalities. Teams striving to achieve a lot of great output in a short amount...