We narrowed to 133 results for: 11

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Oct. 4, 2016, 2:30 p.m.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

March 21, 2024, 1:15 p.m.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

June 1, 2023, 1:15 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

Learn about fluorescent protein techniques you can use to monitor cellular trafficking throughout an organism.

Aug. 15, 2017, 1:24 p.m.

Learn how to target donor and acceptor fluorophores for FRET experiments.

July 19, 2016, 2:30 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Prime editing is a search-and-replace CRISPR editing method that offers versatility and precision in genome editing. We cover common prime editing tools.

Aug. 11, 2026, 1:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.