...researchers from different locations. Having virtual face-to-face meetings as opposed to phone calls most closely...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...Scientific conferences and workshops are another great place to meet up with collaborators. Being able to present...

...These scaffolds are stretches of non-coding RNA interspaced with linkers that can fold into a secondary structure...this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit...five genes that encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering ...generate differently pigmented derivatives of violacein. Using dCas9 as an on/off switch The multiple ...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed...cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the homogenate and...shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate...

...the secretory pathway to their subcellular or extracellular destination. At the end of their life cycle,...to be a part of an organelle or involved in intracellular transport pathways, it is possible to map the... in order for the antibodies to reach their intracellular targets. Therefore, it can be beneficial to ...Transfer, FRET. Additional opportunities: Tracking intracellular pathogens In addition to tracking the location...possible to follow the life cycle of viruses and intracellular bacteria – have a look at our Microbiology resources...

... enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target...SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used the library to identify RBD...RBD mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV...

...to almost every person regardless of profession, race, or gender. Almost no other disease receives as ...divide only at the right times and in the right places. Checks and balances: protecting against cancerous...Luckily, there are several safety mechanisms in place to protect us from rogue cells when something does...variety of cancers, Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult...Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer Lineage Tracing ...

...volunteering, it gave me a place to go once a week to get recharged. The fun change of pace helped break up the... great way to share some of the research taking place at our university with the community. I even spray...interact with their followers. It’s also been a great place to reach out to local establishments such as museums...provide plenty of negativity, so I volunteered at a place that made me happy. I started volunteering in the...

...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...school’s Math & Science Night. There are plenty of places to get ideas for science activities. I did some...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation... considered proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but.... Maybe you just like to see the look on a kid’s face when you’ve shown them how to isolate DNA from a...

...REPAIR (RNA editing for programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated ...they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase off-target...

...your science, one of the big questions you'll be faced with is "how?" How are you going convey your information...learned (somewhat successfully) how to text or use Facebook. However you choose to communicate your science...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

...Production Recombinant antibody production can take place in bacterial, yeast, plant, or mammalian cells. ...variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel...does not, be sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction...10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to ...characterization & rheology, Current Opinion in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449...

...AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed...pediatric population. Once ~400 cases had amassed in places around the world, hypotheses began to develop that...AdV genome in them, including the E1 region). The space in the viral particle’s capacity that is made available... G. R. (2015). Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6)...

...commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as a form of “...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...sequence for Cas9, is located at the 3’ end of the spacer sequence and consists of a single A, U, or C base...each DNA target have high sequence identity to the spacer sequence and contain the appropriate PAM sequence...cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides in length and does not require...

...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...mutations wouldn’t matter – the mutated copy could be replaced by simply dragging and dropping in a functional...allowing a one-size-fits-all approach to gene replacement therapy for most patients. But how does one go...chance of the insert being “dropped” in the wrong place, while increasing the number of potential applications...

...vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. ...low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer... rabies glycoprotein now serves as a retrograde tracer so that scientists can identify the neurons that...2001).  This method can also allow scientists to trace a neuron’s projections back to the cell body. Rabies...

... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...thoughts on why we decided to do an AMA in the first place and give you some reasons why you should consider...realized that the r/Science AMA series was the best place for us. We’re definitely glad we did - you can see...lot of links and content at hand in three useful places: I had posted content on many of the requested...

...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses...ideal behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting...hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test...

... to you: our first, second, and third place winners!  1st Place: Krampus Saves Christmas! created by the... Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team...Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by Joanne Jary from Lab ...

...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification was impossible...light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics As with many TALEN-based... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...