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We narrowed to 610 results for: ACE;

Showing: 121 - 140 of 610 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...interesting, much of what a phage faces as it approaches a host cell surface are complex polysaccharide structures...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...colonies overnight), flip the membrane over and place it colony-side up onto a fresh LB plate containing... is done, peel the membranes off the plates and place each onto a lysis solution-soaked filter paper (... blocking solution (around 108 CFU/mL is a good place to start) and add your membranes to the solution...

  2. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...your Addgene account.  Orders do not need to be placed immediately upon request approval. Once approved...request will remain in your account until you have placed your order. You will only be charged once you have...  What happens after I order?  Once an order is placed, the Addgene viral vectors team gets to work! They... before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for the service... you’ll be able to work with the distributor to place a request and order if approved.  I want to stay...

  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only ...It’s part of Google Drive so it has a similar interface as Google Docs or other products in the suite....collection and drag and drop an icon onto the workspace. The color and size of icons are adjustable. New...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...researchers from different locations. Having virtual face-to-face meetings as opposed to phone calls most closely...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...Scientific conferences and workshops are another great place to meet up with collaborators. Being able to present...

  5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...These scaffolds are stretches of non-coding RNA interspaced with linkers that can fold into a secondary structure...this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit...five genes that encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering ...generate differently pigmented derivatives of violacein. Using dCas9 as an on/off switch The multiple ...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

  6. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed...cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the homogenate and...shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate...

  8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...the secretory pathway to their subcellular or extracellular destination. At the end of their life cycle,...to be a part of an organelle or involved in intracellular transport pathways, it is possible to map the... in order for the antibodies to reach their intracellular targets. Therefore, it can be beneficial to ...Transfer, FRET. Additional opportunities: Tracking intracellular pathogens In addition to tracking the location...possible to follow the life cycle of viruses and intracellular bacteria – have a look at our Microbiology resources...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target...SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used the library to identify RBD...RBD mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV...

  10. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...to almost every person regardless of profession, race, or gender. Almost no other disease receives as ...divide only at the right times and in the right places. Checks and balances: protecting against cancerous...Luckily, there are several safety mechanisms in place to protect us from rogue cells when something does...variety of cancers, Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult...Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer Lineage Tracing ...

  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...volunteering, it gave me a place to go once a week to get recharged. The fun change of pace helped break up the... great way to share some of the research taking place at our university with the community. I even spray...interact with their followers. It’s also been a great place to reach out to local establishments such as museums...provide plenty of negativity, so I volunteered at a place that made me happy. I started volunteering in the...

  12. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed...pediatric population. Once ~400 cases had amassed in places around the world, hypotheses began to develop that...AdV genome in them, including the E1 region). The space in the viral particle’s capacity that is made available... G. R. (2015). Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6)...

  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...school’s Math & Science Night. There are plenty of places to get ideas for science activities. I did some...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation... considered proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but.... Maybe you just like to see the look on a kid’s face when you’ve shown them how to isolate DNA from a...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...REPAIR (RNA editing for programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated ...they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase off-target...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...your science, one of the big questions you'll be faced with is "how?" How are you going convey your information...learned (somewhat successfully) how to text or use Facebook. However you choose to communicate your science...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Production Recombinant antibody production can take place in bacterial, yeast, plant, or mammalian cells. ...variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel...does not, be sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction...10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to ...characterization & rheology, Current Opinion in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449...

  17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as a form of “...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...sequence for Cas9, is located at the 3’ end of the spacer sequence and consists of a single A, U, or C base...each DNA target have high sequence identity to the spacer sequence and contain the appropriate PAM sequence...cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides in length and does not require...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...mutations wouldn’t matter – the mutated copy could be replaced by simply dragging and dropping in a functional...allowing a one-size-fits-all approach to gene replacement therapy for most patients. But how does one go...chance of the insert being “dropped” in the wrong place, while increasing the number of potential applications...

  19. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. ...low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer... rabies glycoprotein now serves as a retrograde tracer so that scientists can identify the neurons that...2001).  This method can also allow scientists to trace a neuron’s projections back to the cell body. Rabies...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...thoughts on why we decided to do an AMA in the first place and give you some reasons why you should consider...realized that the r/Science AMA series was the best place for us. We’re definitely glad we did - you can see...lot of links and content at hand in three useful places: I had posted content on many of the requested...
Showing: 121 - 140 of 610 results