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  2. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...

  3. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...

  7. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

  8. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...

  11. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...

  12. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember” which...protein-mediated cues control polarity during planarian regeneration." Developmental biology 339.1 (2010...

  15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...homodimerization is characterized by an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

  16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

  17. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...

  20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...
Showing: 121 - 140 of 319 results