We narrowed to 514 results for: Cit

...features and limitations of existing backbones, we're excited to share our very own set of empty, HA-, and EGFP-expressing...TurboCas improves on these in both efficiency and specificity. With TurboCas, the Ali Shilatifard Lab was able...characterized these tools and validated their specificity in mice, non-human primates, and human tissue...layers (L1-6) and scale bar are indicated. B) Specificity, as percentage of BiPVe3-dTomato and PV-positive...

... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...Messing, A., & Brenner, M. (2004). Expression specificity of GFAP transgenes. Neurochemical Research, 29...MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene Expression in AAV-Mediated...

...itself, please read on to learn more about this exciting technology from the folks at Transposagen. This...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...

...possible to control neuronal activity with a specificity far greater than the one that can be achieved...channelrhodopsins that, in turn, trigger neural excitation, whereas yellow light activates halorhodopsins...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development: the ...

...important to note that naked and unmodified dsRNA elicits immunogenic reactions that complicate the effectiveness...the most prevalent method due to ease of use and capacity to generate a long-term RNAi phenotype. Long hairpin...transitivity,” calls into question any claims about specificity of a given RNAi technique. While transitivity...Steinmetz LM, Drewes G, Huber W (2019) Biological plasticity rescues target activity in CRISPR knock outs....

...stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other...limited to only a few kb so attaining neuronal specificity through promoter-directed expression alone is...generic promoter). A DIO opsin allows cell type specificity dependent on the promoter of the cre recombinase...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...

...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ... kit pages as well! Some kits that may not be explicitly labeled as “yeast”, “transfection-grade”, or ...

...We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ...it be nice if those weeks and months of work was cited by others?)  Ready to submit?  Fantastic! The next...

...hospitalization for gynaecologic disorders” in Ghana. We’re excited to see what impact Dr. Bengyella’s work will have... chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass...Seeding Labs’ Instrumental Access program. We are excited to discover what new and creative ideas applicants...

...scientists using Addgene in their labs. And it has been exciting to learn about all the amazing research being ...neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT...community. The scientists we've spoken with are excited and passionate about making such tools open-access...

...plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch...importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used...its ever-evolving needs. For now, we have many exciting things planned in 2023, from the expansion of ...

...can now assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting...changing certain amino acids you can change the specificity,” explained Zhanar Abil of the University of ...sequence. By comparison, Abil explained, the specificity of other RNA binding domains depends on the secondary...

...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...introduction to pCri, please see the original publication cited below. pCri plasmids are available either individually...

...accepted that day and was excited for the start of my co-op in January. This excitement did not come without...hopeful to continue on in the process and I was excited when I received an email to invite me back to conduct...

...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... Packaged on Request viral vector prep.    Excited? So are we! This expansion will give researchers... and educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging...

...fluorescent protein to create three colors. The specificity of these orthologs is key: due to differences...illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential imaging...useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References Multicolor...

...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

...cell activity, enables researchers to silence or incite neuronal firing and study subsequent effects on...swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups were activated...illuminated. By looking at groups of neurons that elicited similar tail movements, they were able to narrow...

...reagents along with clear documentation, so we were excited when Amos Bairoch from the Swiss Institute of Bioinformatics...going through the pdfs of the 26,000 publications cited. If the information seems incomplete or unclear,...line distributor. A success all around! If you’re excited about this resource and want to learn more, check...

...") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle...chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects....done with CRISPR technology in just 2 years and excitement about how much more there is left to do with ...