We narrowed to 181 results for: DAP

1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

2. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

3. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...

4. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

8. Hot Plasmids February 2024

   Type

   Blog Post

   Published

   Feb. 20, 2024, 2:45 p.m.

   ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

9. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   Published

   April 18, 2024, 1:15 p.m.

   ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

10. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

11. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

13. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

15. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., (2018).   HaloTag The HaloTag...

16. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

17. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

18. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

20. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes...