We narrowed to 379 results for: ENA;
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TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid...
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Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....what MoClo is and what types of experiments it enables. What is modular cloning? Modular cloning, or MoClo...gene, and terminator. Fig 1. MoClo enables individual parts to be assembled into larger functional... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..../10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power...Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical Additional resources on the Addgene... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....Institute (Harvard University), we developed two new enabling technologies for the field of synthetic biology... proof-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m.... Haeussler. With a few extra lines of code, we enabled it to return guides and primers in batch, optimized...Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m...., which sit on the surface of coronaviruses and enable the virus to enter host cells. To do this, they... lab developed a robust in-vitro protocol that enables rapid selection of synthetic nanobodies, called... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....optogenetic or fluorescent tool with magnets would enable new research and applications that don’t yet exist...Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M. K., Deppmann, C. D., & Güler, A.... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....This approach can augment viral surveillance by enabling rapid assessment of antigenic drift. Starr...rate-limiting enzyme in this pathway (myo-inositol oxygenase), providing enzyme protection while still allowing... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....that nearly all of the cell lines we tested were amenable to cloning in this manner, we once had difficulty...have prepared two 48-well plates of clones) which enables investigators to identify specific clones with ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....” transistor-like digital genetic switches that enable cellular computing. These logic gates built from...available via Addgene and the BPA Plasmid Collection - enable? Endy: The BIOFAB collection is the beginnings ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....ease with which BiFC can be visualized makes it amenable to studying PPIs under relatively physiological...dissociation of PIPs. Thus, whereas BiFC is useful in scenarios that call for a simple, yes-no answer — i.e., ... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The addition of RESCUE further.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....bacteria and denature the DNA and proteins in solution. The pH is then lowered using a renaturing solution... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation method...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand... Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about Genome-Wide... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m.... which may also induce cell death. CRISPR also enables increased specificity - rather than targeting a...antibiotics, but the field is currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... of such engineered DNA-binding molecules even enables us to skip the step of knocking-in. In 2013, we...dCas9 and guide RNA. The retroviral enChIP system enables us to express the CRISPR complex much easier in... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m.... with DNA amplification in the host cell. This enables relatively high and long-term expression of the... ml of starting blood sample, in the best-case scenario [7]. In the clinical setting where patient blood... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban... Ban Asbestos Now Act of 2016 in the U.S. Senate. If this bill passes, it will put an immediate stop to... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m.... courses of treatment with antibiotics These scenarios expose bacterial populations to antibiotics at...counter-intuitive considering the other three scenarios, but a short treatment regiment might wipe out...