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We narrowed to 450 results for: HA tag

Showing: 121 - 140 of 450 results

  1. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) 121538  AAV5  pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 119741  AAV5 ... vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato, pAAV-GFAP104...iGABASnFR GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G...its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical activity in...measured with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical...Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent version...pAAV-hSyn-EGFP *Also coming in our new 20 ul trial size! Voltage indicators Plasmid Serotype Name 119036  AAV1...

  2. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...pair changes, and the addition of protein tags. It also has the flexibility to modify the E. coli chromosome...non-selectable DNA fragments, such as gene replacements and tags.  The typical frequency of recombinants is 1 positive...recombineering system The lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and... in the center of the sequence. Since lambda red has a higher recombination frequency when the lagging...activating MMR: 1) use a strain of bacteria that has key MMR proteins knocked out or 2) specially design...recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first be selected...repress expression of exo, beta and gam.  A major advantage of using this method for lambda red expression...

  3. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...Plasmids Find Empty Backbones with Fluorescent Protein Tags  ... first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently expressed...using chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. ...Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539 ...for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful...121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  ... AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1...

  5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest... dronpa fusions. With these various designs, Xin has been able to develop photoswitchable Intersectin,...Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety... of cells that actively produce the HMOs. Fatima has demonstrated the successful production of both types...precisely spatio-temporally controlled as a key advantage over other inducible enzyme technologies. She ...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...surprising discovery that both purified CPP-tagged protein and an untagged control could enter cultured cells ...derived from the same cell type; however, their usage has been limited by technical problems. For instance,...binary systems in which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated...effects. Third, protein purification (once a protocol has been established) may be less laborious than viral...used for protein purification must be helping the untagged protein enter cells. Subsequent work found that...stem cells, they found very high recombination percentages (78-79% after two rounds of transduction.) Compared...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...cells according to whether or not they have been tagged with a fluorescent protein or dye. It separates... distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3.... cell mobility within these complex environments has never been easy and, for many years, researchers ...Intravital imaging powered by two-photon microscopy has uncovered host-pathogen interactions leading to the...proteins can be used in your experiments, experience has shown that some are more reliable and more efficient...important to finely tune the excitation wavelength as it has been shown that even a small incremental change of...this system called Brainbow, the Ubow mouse strain has been created to fate map Langerhans cells and follicular...

  8. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...into a vector with a large tag at the N-terminus, but you don’t have the tag already included in the vector...you can insert both the gene of interest and the tag sequences into the vector in one step without scars...where the vector that you want to use is very large, has a high GC content, contains a lot of repeats--any...In this blog post, I will go over some advantages, disadvantages, and examples of how scientists are using... common, and for good reason. They offer many advantages over the traditional restriction enzyme cloning...thereof) nor a serious time commitment. Another advantage is that this process makes it easy to generate...will be using. Some of these products may offer advantages that Gibson assembly does not such as increased...

  9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... be selected, since it cleaves at the stop codon TAG. Design your repair template with 55-100 base...mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N terminal); 6xHis (N ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...respectively. mNeonGreen can be used as a fusion tag for a wide range of applications from traditional...single-molecule super resolution imaging. mNeonGreen also has little sequence identity to other common fluorescent...fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks (max excitation...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

  11. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within minutes, but requires ...being targeted via RNAi. Recently the Vellai lab has created an improved RNAi vector that leads to significantly...sequence of the toehold switch. For example, Green has also developed toehold switches to detect Zika virus...protein motifs than previously thought. Sidhu’s group has made several hundred SH3 domain containing plasmids.... In addition to these constructs, the Sidhu lab has made hundreds of additional plasmids available through...infect millions annually. Because it is highly contagious, it is essential to contain outbreaks when they...

  12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good signal...encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. ...Technology (KAIST) and Institute for Basic Science (IBS) has developed another useful optogenetic tool that enables...facilitate future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic...operon originates from Pseudomonas putida CBB5 and has been tailored for expression in E. coli. This operon... contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors, ShadowG, a... due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption than ...

  13. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... expressing fluorescent proteins will be used to tag individual strains of bacteria. Their interactions... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

  14. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our hope that by...Good bye 2020! What a year it has been for all of us. As we eagerly await 2021, we've looked back on ...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...elements – promoters, origins of replication, protein tags, and antibiotic resistance markers (just to name...restricted based on experimental constraints. This has been used for a wide range of purposes, including...Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce Cre into cells... can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for...recombination at the loxP recognition sites. A key advantage of the system is that Cre acts independently of...in certain cell or tissue types, during certain stages of development, or by making the Cre inducible ...

  16. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... purified them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring... characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent protein... from R1 institutions to community colleges, and has helped provide original research experiences for ...

  17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified...same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator fusion proteins. The...which one of Cas9’s two critical enzymatic residues has been converted to an alanine (D10A or H840A), changing... for your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and...looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a...SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020). Further, this...reverse transcriptase, and a nCas9. While this system has been shown to be less efficient than base editing...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...your experiment. For example, does the fluorescent tag need to be pH stable or withstand a particular solute...enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small...cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally follow...

  20. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...integrins, and more coming soon) as well as epitope tags like His, Flag, and Biotin. The antibodies developed...%}{% end_module_attribute %}{% module_attribute "tag" is_json="true" %}{% raw %}"module"{% endraw %}{%...complicated math. Bioengineering is a field that has been gaining a lot of traction in recent years. The... wealth of smaller engineered antibody fragments has further increased possibilities for synthetic antibody...coupled with iterative rounds of selection and mutagenesis to refine antibody performance. If you are interested...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology...
Showing: 121 - 140 of 450 results