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We narrowed to 809 results for: LAS

Showing: 121 - 140 of 809 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...you encounter mycoplasma contamination. Causes of mycoplasma contamination The mycoplasmas enter the cell...removal of mycoplasma but, can get rid of most of mycoplasma contamination and are the last alternative... growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation...suppress the growth of mycoplasma but cannot eradicate it completely. When mycoplasmas are seen in the culture...highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes or optical microscope...most of the mycoplasma species, apart from a few of them. Another method used for mycoplasma detection ...infecting mycoplasmas are used. A broad range of primers encompasses almost all the infecting mycoplasma. The...

  2. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important...of purity. Based on these premises we can classify a plasmid preparation in 3 different ways: Transformation... into a plasmid backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian... an external plasmid in mammalian cells and it will likely require micrograms of plasmid DNA for each ...larger scale, to allow the extraction of enough plasmid to last for multiple experiments. Another fundamental...utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation ...Addgene blog Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during plasmid DNA ... a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...Does it make a difference? Short answer: yes. This last step will influence the stability of your sample...Nowadays, the most common E. coli strains used for plasmid amplification are endA negative, meaning the gene...degradation and which experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water...polymerases. In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective ...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...viral vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...contain the full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb...in my samples? Plasmid recombination can affect viral vectors (LTRs and ITRs), plasmids with multiple ...other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids and ... length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid and the...smaller, recombined plasmid around 1.5 kb. When cut with BamHI, the expected linear plasmid is present, but...get to see everything that can go wrong with a plasmid. One of the most frequent problems that I help ...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...30, 2020 and last updated Jul 12, 2021. What started out as a small collection of plasmids for coronavirus...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...scientists through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the...

  6. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...individual lentiGuide gRNA plasmids targeting the human kinome. These plasmids contain the same 3-4 gRNAs...true biological insight. Pooled and individual plasmid formats A first for a library at Addgene, you can...first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes, ... screening in a pooled setting. The individual plasmids will also be useful for imaging or gene expression...assays necessitating the screening of individual plasmids separately. Additionally, this set can be used... Page Find Validated gRNAs Deposit Your CRISPR Plasmids ...

  7. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing... on our plasmid pages - links to articles citing this plasmid. Now you can learn how a plasmid has been...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested...Citing This Plasmid" list. Email us at [email protected] with the Plasmid Name, Addgene Plasmid ID #, and...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...explore the TetO-FUW-OKSM plasmid page. Or search for your favorite plasmids and see where they've been...

  8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...Addgene through the Biobrick Public Agreement (BPA) Plasmid Collection along with a BIOFAB kit comprised of..."Addgene is one of the leaders in distributing plasmids amongst the academic community. On the BioBricks... with Endy more about the BIOFAB and BIL gates plasmid kits, the Biobricks Public Agreement, and the future...first to be made available via Addgene and the BPA Plasmid Collection - enable? Endy: The BIOFAB collection...engineering an entire cell. Typically when people use plasmids, they are trying to do one thing at a time. What... tricky to tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... Read other Plasmids 101 blog posts New to plasmids? Read our article on What is a Plasmid? Download the... the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find vector...

  10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple...the overexpression plasmids. Finally, students determine if the presence of the plasmid corrects any known...diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long research...less-well studied organism into overexpression plasmids. Students next transform deletion strains from...phenotypic abnormalities due to the deletion (i.e. the plasmid complements the deletion). If the phenotype is ...rearrangements in the S. cerevisiae lineage (7). In our class research project, students transform S. cerevisiae...cerevisiae met deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or ...

  11. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ... we use SnapGene for plasmid quality control are: To identify important plasmid features To align NGS...design experiments for further plasmid verification Identifying plasmid features While our quality control...control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always...send the plasmid off for Sanger sequencing.     If a repetitive region is present in the plasmid, we might...sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps and sequences...the Addgene Blog How to verify your plasmid Check out our Plasmids 101 featured topic page Additioinal...results to perform quality control on deposited plasmids. We’ve also talked about how our new Snapgene ...

  12. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests...paper has between 1–32 plasmids and a total of 2–2,590 requests. We received plasmid requests from 56 countries...Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests...number of plasmids ordered for each paper. Rough estimation of COVID-19 effect on plasmid requests ...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request activity...of how plasmid orders were affected by the COVID-19 pandemic, we compared the number of plasmid orders...Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find plasmids from David Liu's...

  13. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...Ginkgo Bioworks COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available ...COVID-19 plasmids to industry scientists in the short term, did you know we have other plasmid collections...organizations have made their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox...and plasmids from several labs at Florida State University became openly available. Find plasmids available... more about the Ginkgo COVID-19 plasmid collection Read about plasmids in the COVID-19 collection ...one critical part was missing when the COVID-19 plasmids first became available: distribution was limited...limited to academic scientists. Expanding plasmid sharing during COVID-19 Realizing this limitation, we ...

  14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...fragments. This system has been used to construct plasmids for use in model organisms such as E. coli and...

  15. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    Published
    Feb. 6, 2025, 2:15 p.m.
    ...temporary and now on a more lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ... Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...

  16. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to ... has been working with Seeding Labs to provide plasmid grants to researchers in developing countries. ... to announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena...doctors Bengyella and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis ...uterine tumors. According to Dr. Bengyella, this class of tumors is “a major public health problem, and...cloning methods and tools to construct chimeric cellulases that enable E. coli and Citrobacter freundii ...cellulosic biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular...

  17. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...about Global Plasmid Distribution Check out the List of Institutions that Deposit their Plasmids with Addgene...community and are always taking steps to increase plasmid distribution and disseminate knowledge around the... we will be working with Seeding Labs to award plasmids to research labs that have received Instrumental...Seeding Labs and that can demonstrate that these plasmids will effectively advance their research. The ...below shows all the countries that have requested plasmids from Addgene since our inception in 2004. In the...eliminate much of white space on this map and increase plasmid sharing across the globe! We hope that these awards...

  18. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    Published
    Dec. 8, 2015, 3:30 p.m.
    ...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on...Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in the Cepko Lab Listen to...Addgene Website Find Cepko Lab Plasmids at Addgene ... plasmid tools...

  19. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode! Expanding.... Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid as well as several...further probe cellular function. The CRY2clust Plasmid can be found here. Park et al. Nat Commun. 2017..., the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic operon that enables the ...constitutive promoter (BBa_J23100) and expresses N-demethylases and other required enzymes, including a glutathione...

  20. Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

    Type
    Blog Post
    Published
    Dec. 11, 2017, 2:02 p.m.
    ... this Hot Plasmids episode! Hot plasmids covered in this segment Hot Plasmid 1: B. subtilis knock out ... find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage. Listen to this...In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying...studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can... Our segment on the B. subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming... segment on modulating iPSC reprogramming  Hot Plasmid 3: Regulating telomere length with TZAP. Listen...segment on regulating telomere length with TZAP Hot Plasmid 4: CRISPR tools for use in plants.  Listen to the...
Showing: 121 - 140 of 809 results