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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 463 results for: MEA

Showing: 121 - 140 of 463 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...abilities, or other phenotypes that can be efficiently measured at the single cell level), are separated by, for...mouse libraries available through Addgene. This means you could perform a screen with one library from...can be used: Design a screen with an easily measureable cellular phenotype (e.g. survival of a cancer...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...GPCRs in the human genome are orphan receptors meaning their ligands are unknown. Identifying new ligands...Protein A tag. The luciferase activities can then be measured and normalized to assess interactions. Higher ...of CRISPR and AID technologies provides a clever means of investigating hard-to-study genes in hard-to-... work, they used the CRISPR/Cas9 technology to measure the contribution of each gene from the model apicomplexan...

  5. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked with”...

  6. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...this new green glowing protein could be used to measure gene transcription, as well as to track protein...

  7. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...in neurons. Indeed, this AAV provides you with a means to specifically activate expression of any Cre-dependent...Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals...

  8. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...on the team talks and listens in roughly equal measure, keeping contribution short and sweet. One key...team. We are not talking about gossip here – this means encouraging a culture of maximum information flow...

  9. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...Addgene nearly 10 years ago, lunch felt like a family meal. My co-founders actually were my family (Kenneth... forward to gathering around a large table for a meal with my family. I am thankful for many things, and...

  10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... protein shell that excludes water but is gas permeable. These vesicles are primarily used by water-dwelling... of arg1 and arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially...

  11. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...significant being the usage of a strong detergent to permeabilize cells rather than to rely on nuclei extraction...desired. It also provides an excellent additional measure of DNA binding sites in combination with existing...

  14. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ... 1 out of 10,000 cells on average! Without some means of quickly determining which cells successfully ...Polymyxin B polypeptide Alters outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...other uses. Many laypeople would use “PCR test” to mean “a test that uses a biological sample to look for...for presence of a virus.” A scientist would likely mean “a test for the presence or absence of a predefined...

  17. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ...

  20. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...interest, the team could visualize three loci and measure inter-locus distances. They tested this system ...intergenic DNA region (IDR3) as a reference loci to measure inter-locus distances of loci pairs from intergenic...
Showing: 121 - 140 of 463 results