We narrowed to 632 results for: Ncl

...per gene and includes ~10,000 gRNAs targeting intergenic regions. The libraries also include 2,400 gRNAs...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

...replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require... same features annotated in our plasmid maps, including our most popular primers and certain restriction...

...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

...for half of the trip. Preparations ahead of time included contacting YNP staff to arrange permits to visit...frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box to monitor...route that would reach all of the remote areas, including one hot spring requiring an overnight hike in....

...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

...structure and transparency of methods sections, including clear information about reagents, and why other...roles on review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, ...inception and launch of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September...

..., Musicophilia includes case studies of relevant neurological atypicalities, including a patient with ...protagonist, some clever magic, and a satisfying conclusion? In this series, there is always another secret...

...biological processes is highly useful in other fields, including synthetic biology. Addgene depositors Christopher...effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity ...they can be used with many types of proteins, including Cas9 and dCas9-transcription factors, for which...

...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...