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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 121 - 140 of 628 results

  1. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in celebration of ...trouble, even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...review. 8)     That same issue of Science also included an online survey of scientists showing overwhelming...

  2. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is...people for years and have gathered much acclaim - including being featured in the National Geographic and ... are now taking place and gathering momentum, including in Cambridge and Bristol. If you’re a scientist...

  3. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...team reveal the mechanism behind its activity including an extensive RNA scaffold and a domain needed ...inhibition library from the Chong Zhong lab. Now, we’ve included another E. coli genome-wide inhibition library...recently deposited to Addgene and you’d like it to be included in the next What’s New in CRISPR blog post, please...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...don't read, you're doomed.” 2. Always be sure to include various positive and negative controls to “convince...entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped away from...Blog Series   Our 3 PIs answer more questions, including: Advice for Choosing a Research Project Finding...

  5. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require... same features annotated in our plasmid maps, including our most popular primers and certain restriction...

  6. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...straightforward in cases where the inserted DNA includes an antibiotic resistance marker. After transformation...grow. However, often times, the plasmid backbone includes an antibiotic resistance cassette and you aren...Gateway cloning takes advantage of this property by including ccdB on its destination plasmids. In the destination...

  7. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...years of study to come. Some Freshers Fairs can include fairground rides and other fun activities and even...society organises talks by prominent scientists, including Nobel Laureates, who speak about their work and...recreational and educational events throughout the year, including a Christmas Chemistry symposium and a career night...

  8. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...thickness. The fibers are drawn with microelectronics included in them for downstream control – this is important...components are added - the ‘bells and whistles’ – including microscale light emitting diodes, thermal sensors...neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar neural fiber...

  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...purification (see plasmid map and cartoon below). Including biotin in your culture and inducing protein expression...binding kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences...

  11. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

  12. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

  14. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...structure and transparency of methods sections, including clear information about reagents, and why other...roles on review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, ...inception and launch of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September...

  15. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., Musicophilia includes case studies of relevant neurological atypicalities, including a patient with ...protagonist, some clever magic, and a satisfying conclusion? In this series, there is always another secret...

  16. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

  17. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...biological processes is highly useful in other fields, including synthetic biology. Addgene depositors Christopher...effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity ...they can be used with many types of proteins, including Cas9 and dCas9-transcription factors, for which...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...genome. Typically, it is the sequences between and including the LTRs that are integrated into the host genome...internal promoter. An internal promoter must be included as the wildtype LTR promoter is no longer present...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  20. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...will it make them think? Does this lead to the conclusions I want them to draw and the perspective I want...scientists has formed around science communication, including the teams at Addgene, ComSciCon, and many more...
Showing: 121 - 140 of 628 results