We narrowed to 298 results for: Nov
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TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....through your proposal, ask yourself: is it clear how novel my findings will be and why these findings will ...problem can allow you to approach that problem in a novel way. Finally, engineering new applications to solve...
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5 Tips for Troubleshooting Viral Transductions
TypeBlog PostPublishedAug. 11, 2016, 2:23 p.m....Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions for storage...Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus? Check out Our ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....Find the allelic exchange vectors here! ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used...A., Koga, N., & Nabekura, J. (2019). ShadowR: a novel chromoprotein with reduced non-specific binding ... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, New England Biolabs, Regeneron...pre-approved project. Other companies, such as Novartis, have specific web sites for postdoc listings,... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m..... https://doi.org/10.1074/jbc.RA120.01289 Novel protein cage provides simple enzyme protection in...Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins in yeast... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics...Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....) system functions by harnessing the power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing...can be degraded by this selective degrader. The novel degrader, dTAG13, is a small molecule which induces... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can form a complex with... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....incubation can be skipped by pre-binding to 1° Monovalent Monoclonal Only anti- rabbit and -mouse versions...Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....ligands. New fluorophores As a corollary to these innovative labeling strategies, several groups—including...infusion of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....choice. ThermoFischers GeneArt Gene Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz...Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out Addgene'... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....mutation or morpholino. This observation unlocked novel research into genetic compensation, a previously...otherwise normal cell types are produced, rather than novel, abnormal cell types. Sequencing-based lineage tracing... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....program should “enable the ability to study life’s innovations from anywhere in the tree of life.” Accepted ... biomass). The lab is developing a system using novel vesicles to efficiently transform these cells which... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m.... by our partners at the Institute for Protein Innovation (IPI), part of a new collection of epitope tag...Aryana Khosravani Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test, the authors...show that HR blocked neuronal activation in the novel context as determined by reduced c-fos expression... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....19 testing methods. Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR...for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....capable of inducing the brightest fluorescence (Filonov et al., 2014). Broccoli is twice as bright as Spinach2...9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....adeno-associated virus, herpesviruses, hepatitis B virus, adenoviruses, and human papillomavirus (HPV), to name a few...though the rate of loss greatly depends on cell turnover. Why use integrase-deficient lentiviral vectors... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....e.g. mScarlet) or infrared fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer...24360272. PubMed Central PMCID: PMC3918502. 14. Chernov, Konstantin G., et al. "Near-infrared fluorescent...