We narrowed to 228 results for: Ott;

1. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about patients As ...

2. Components of CRISPR/Cas9

   Type

   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ...target sequences specific to each individual crRNA. Bottom line, the “direct repeat region” combined with ...

3. Management for Scientists: Delegating is Key

   Type

   Blog Post

   Published

   Feb. 20, 2015, 8:30 p.m.

   ...Joanne's Reddit AMA   Benefits of delegating The bottom line – delegation allows you to get more done. ...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...of a Manager by Julie Zhuo Radical Candor by Kim Scott Courses: Leadership programs on platforms like...

5. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...though it’s hard to call from this plot. The red (bottom) peak overlaps with the baseline suggesting this...

6. Clear and Accessible Writing

   Type

   Blog Post

   Published

   Feb. 15, 2024, 2:15 p.m.

   ...enter the column so the virus will flow out the bottom syringe."Feel free to share your alt text in the...

7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...don’t benefit from this approach. One central bottleneck is that most recurrently mutated cancer genes...

8. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies Differences ...

9. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ...pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey...

10. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...tracking, immunolabeling of fixed cells, Western blotting, and more. Given the popularity of FLAG and HA...

11. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ...digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why...

12. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double...

13. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...most popular movie in theaters, the Razr was the hottest phone on the market, and two new websites, Facebook...

14. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...

15. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...happens to other people, it’s normal and quickly forgotten, and when it happens to you, it’s noteworthy and...

16. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...frustration —> increasing the happiness. My unofficial motto in my Product Manager role at Addgene is “Words ...

17. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

18. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...ensure lack of degradation in your context. Immunoblotting is recommended, while quantitative proteomics...

19. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ....1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel Z (2019) ...

20. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...on the Addgene blog The Basics of Western blottingAntibodies 101: Introduction to AntibodiesAntibodies...