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We narrowed to 488 results for: Ret;

Showing: 121 - 140 of 488 results

  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...developed a split intein spCas9-AAV toolbox that retains the gene-targeting capabilities of full-length ...10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

  3. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...time to start answering questions. r/Science has pretty strict rules about not being too sales-y in posts...questions from the AMA - as you’ll see below, it pretty nicely displays her energetic, truthful and helpful...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...support, but there are eight other teams who still retain a customer support element amongst their other ...The first and foremost sub-skill to have here is pretty intuitive: You need to be willing to face conflict...this skill to another job, it will be important to retain the stories and examples that have guided your ...

  5. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...get them to work on DNA instead. However, they retained a small amount of RNA-editing activity, which ...reduced RNA editing to background levels while retaining acceptable on-target DNA editing activity. In ...References Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more recently used by Stephen...Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and Opto-hRET – as well as various LOV-domain-mVenus constructs...directionally assemble in a single reaction, while retaining only a defined 4bp fusion site in between. The...vectors with the desired sequence of interest are retained after SLIC cloning. The general cloning strategy...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...copper cathode/iblot gel matrix, and filter paper. Pretty much like an actual sandwich! S'mores image from....2015.04.012 Burnette WN (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide....76.7.3116 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels...

  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...experience. Working in industry: Microbiology On returning from Ghana at the beginning of 2010, I explored...looking for other career options after 6 months. Returning to university to study a Masters of Science I ...programming. Working for a scientific nonprofit On returning to the UK, I checked out the job postings from...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...data and can instill greater confidence when interpreting the outcome.    References and resources References...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    .... Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9 MOI considering that...I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short DNA ...cancer cell line in about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research...

  12. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S....

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...Off-target activities of the RNAi Correctly interpreting your data therefore necessitates careful design..., this can make the results more difficult to interpret, and a knockout/ or knockdown approach should ...validation is critical to ensuring accurate interpretation of your results. Therefore, taking advantage...

  19. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is...with Flp-FRT. Additionally, you could use the retrograde form to specifically express Cre in neurons with...

  20. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...toward a more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all...
Showing: 121 - 140 of 488 results