We narrowed to 151 results for: SPEN;
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TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...
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Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....student, I was negotiating with my PI on how to spend my grant money (Can I attend this conference?) and... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....achieve a consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m.... did not lack motivation, but he didn’t want to spend hours reviewing papers to piece together the best... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m...., but with longer homology arms comes more time spent cloning. In contrast, the short homology required... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....protein. Energy and cellular resources are being spent to make something the cell doesn’t need to make.... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....arrive from labs around the globe. The packages have spent days to weeks in transit and some have been transported... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....study inflammation and neurodegeneration. He now spends his time learning and writing about science whenever... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....game changing nature. Full disclosure: Zimmer also spends some time on Addgene’s role in the rapid dissemination... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA is ready for... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....publications in a specific field(s). Most anyone who has spent any time in academia has likely had the opportunity... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV construct... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....have already held. Imagine this situation: You’ve spent hours scouring a company’s LinkedIn pages, given... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....small molecule dyes seems daunting—no one wants to spend their days microinjecting fluorescent conjugates... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....implicated in human diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....seen with the naked eye. In 2023 alone, viewers spent over 22,000 hours watching these and other videos... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....convenient and fast way to deactivate genes without spending months generating mutants. Unexpectedly, phenotypes... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....board before or while the committee is arriving. Spend as much time as possible facing the committee and... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... pellets containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....them demand more services based on what they're spending. Increase the number of science masters programs...