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Addgene

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We narrowed to 490 results for: SUB

Showing: 121 - 140 of 490 results

  1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...expensive exogenously-added substrate, chromoproteins don’t require other substrates for screening. Thus chromoproteins...distinction by name, chromoproteins are actually a subset of the fluorescent protein family. What sets them...

  2. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...number genes may mimic viability genes as they are subject to an increase in dsDNA breaks that can cause cell...al. demonstrated that the Calabrese library substantially identified more hits than the SAM library approach...

  4. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

  5. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then form a blue precipitate. GUS ...Luciferases are great quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...probe gene-function relationships in defined substructures of the CNS as well as individual neuronal populations...demonstrated by the Qi Lab, which developed a subgenome-scale CRISPRa screening approach in embryonic ...transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks to allow differentiation...

  7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...cytokinesis. Why name this protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific... to segmental clusters of the mesoderm. This subdivision of the mesoderm is what gives rise to different...have severely disrupted visceral mesoderm and subsequently the gut does not form properly (Azplazu and ...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... Cas13a binds and cleaves RNA rather than DNA substrates. In terms of structure, Cas13a shares no homology...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ...hundreds of observed shRNA off-targets. They subsequently used catalytically inactive dLwaCas13a as a ...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...textbook. Get the journal’s submission rules for review articles Whether submitting a review by invitation ...for their feedback on the scope and the content. Submit your article! Overall, writing a review can be ...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...output of an initial process as the input to a subsequent process, we linked each step in our pipeline ... build one process at a time and connect each subsequent process to the existing ones. Once our first ...as output, which we specified as input to the subsequent bbmerge process.     Fig. 3: How we get...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... was composed of many protein subunits, it was nonetheless a new subtype. Cas7-11 had combined all the... complex Class I systems that contain multiple subunits, but the more they worked with it, the more they...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...license.    Pro tip! Subtypes of immune cells are often defined by a subset of the markers used to ...you’re interested in studying immunology or subpopulations of cells, you’ll soon find yourself encountering...

  13. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... data is to be made at the time of manuscript submission. The rationale includes increasing the ability...individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent was and provided...be argued various authors have simply chosen to submit elsewhere). The majority of the scientific community...

  14. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...single crossover and integrated into the genome. Subsequent selection on sucrose will identify double crossovers...after about 48 hours, the resulting colonies turn subtly blue or magenta. This is particularly valuable ...

  15. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...a glycoprotein that consists of four identical subunits that can each bind one molecule of biotin. Biotin...surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting...to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith...

  16. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...around the lens. It allowed me to focus on the subject after I’ve left the room. Microphone: Lastly, I...shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just out of frame allowing...camera from a distance if I needed to be close on a subject, or I could manually control the camera by hand...

  17. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...microtubule-destabilizing drugs are impossible to target to specific subcellular regions, laser ablation can damage other cellular...After 30 minutes of blue light illumination, substantial PA accumulates in the plasma membranes, indicated...reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular...

  18. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews.... video interviews. For point of reference, I submitted my applications all by snail mail and only had... me and I'll try to answer your question and subsequently update the ebook. One of my graduate students...

  19. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...hypothesized that the low sensitivity was due to suboptimal folding or stability. Thus they conducted multiple..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...published and unpublished experiments on similar subjects in a single, searchable space. Finally there are...2018). In this ideal situation, a researcher would submit an experimental design to a journal prior to performing...platform for pre-registration wherein researchers can submit designs and transparently reveal them upon completing...
Showing: 121 - 140 of 490 results