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Showing: 121 - 140 of 261 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...Low, S. H., François, A., Suárez-Amarán, L., & Samulski, R. J. (2022). AAV vectors: The Rubik’s cube of...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293 cell culture.... 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production of recombinant adeno-...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...poster or talk at the conference, you can end by asking them to come to your poster or talk. After the ...Volunteer at a professional organization to expand your skill set Resources on the Addgene website Become of...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...Results from a survey conducted by Addgene in 2021 asking scientists what data would improve our AAV Data... your data free. Nature, 5–7. O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure. If you expect a lot of...children but are interested in testing your SciComm skills or are interested in a career in education. Maybe...

  8. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... References and Resources References  Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, ...Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution...

  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...designed with the help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the ...e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...diabetes.55.04.06.db05-0927 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...doi.org/10.3390/cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...10.3390/pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...

  15. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...would improve my scientific and application writing skills, and ended up with the award. We are often more...These applications can be highly competitive, so asking for help from mentors and other students who have...

  17. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon...Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer...

  18. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...this sample type, so you use your bioinformatics skills (or your friendly neighborhood bioinformatician...//doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E...

  19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...complementary edits to opposite strands of the DNA and skip the cellular mismatch repair step (Anzalone et al..., M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L., Zhou, W., Jiang...

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ..., K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P.,...https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg...
Showing: 121 - 140 of 261 results