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We narrowed to 484 results for: Vit

Showing: 121 - 140 of 484 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...infect non-dividing cells is not restricted to in vitro cell culture as lentivirus-derived vectors are capable...promoter of the 5’LTR has been deleted to reduce its activity. A CMV or an EF1α promoter has been inserted in...have to be aware that there is often  background activity and it requires continuous administration of Dox...GFP encoded virus, and the reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of ...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... Cas9 activity in my stable A549 cell pools. Using this virus, it’s expected that Cas9 activity, guided...Cas9 (see tip 2). Pro-Tips 1. To quantify Cas9 activity using this assay, you really need to use FACS ...described in Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Griesbeck, Heim, & Tsien, 1999), monitoring kinase activity (Ni, Titov, & Zhang, 2006), quantifying antibody...structural changes due to this bulky insertion are inevitable. Most FP fusions for FRET studies are at the ...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...strategies. FRET experimental design lends itself to creativity and innovation, valuable commodities in any NIH...

  4. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...tribute to the changing seasons – and they also invited Father Time to the party. They won over our judges...

  5. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...annual Deck the Lab competition drew upon the creativity of the community, who delivered impressive science-themed...In third place is Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time...up at home!This year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com...

  6. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...dual-nuclease Cas9-Cas9 chimeras with higher target site activity and more predictable and precise deletion products...

  7. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ... other projects, using stem cells to develop in vitro models for lung disease. Darrell strives to promote...reliable projects. Chapter 9: Using stem cells as in-vitro models of disease. How Darrell is using stem cells...reagents, cell lines, data, and knowledge without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at...

  8. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  9. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...can we shape our time in academia to maximize productivity and success? We asked leading professors for...suggest that you make time for extracurricular activities both to develop new skills and to discover what...about the benefits of pursuing extracurricular activities. Success evolves from passion True success evolves...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...substrate OR production of a product OR enzyme activity. Small molecule biosensors Addgene has many sensors...constructs are often used to monitor enzymatic activity or phosphorylation over time. You can also use...plate well). Use to track a process (e.g. enzyme activity) over time or under various conditions Localization...

  11. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...include fairground rides and other fun activities and even invite members of the public to attend. We thought...aim to support events and scientific community activities throughout Europe and the world. So get in touch...

  12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...several important shortcomings including low processivity (ie. the probability of the polymerase continuing...heterogeneous RNA samples. Furthermore, such high processivity enzymes and the structural understanding thereof...to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao...

  14. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...available allowing you to chemically control neuronal activity while studying your phenotype of interest. Optogenetic...Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We’re also ...sharing will only help research advance. Remember: productivity is infectious!  Additional Resources on the...

  15. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...resource list available from this post. Use an activity to start a discussion. The Forced Choice Analysis... can use it to clarify and discuss values. The activity is just the start – it is the discussion after...mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips for Choosing...

  16. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...allow researchers to independently control the activity of two populations of neurons at once. Addgene...is a toolset that lets you turn the electrical activity of neurons on or off using pulses of light. By...both activators as well as silencers of neural activity. Since then, there has been a steady stream of...

  19. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar cells and photoreceptors...robust gene delivery into the murine retina upon intravitreal injection." Scientific reports 7 (2017): 45329...

  20. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...we are missing is the unmeasurable skills and productivity coming from that casual discussion on the way...this is an underrated ritual that really drives creativity and novelty in scientific research and in any...springboard for future discussion. Collaboration and creativity are pivotal in writing publications/grants too...
Showing: 121 - 140 of 484 results