We narrowed to 235 results for: bon;

1. Antibodies 101: Epitope Availability

   Type

   Blog Post

   Published

   Jan. 11, 2022, 2 p.m.

   ...using heat and/or chemicals to break some of the bonds keeping the protein folded, changing it from its...

2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   Published

   Aug. 18, 2023, 4:15 p.m.

   ...to a cell but cannot create new particles. As a bonus, this increases the carrying capacity of the viral...

3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions ...

4. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1,...

5. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...pBLADE-mCherry: This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry...

6. Plasmid Cloning by PCR

   Type

   Blog Post

   Published

   March 29, 2016, 2:30 p.m.

   ...MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful to choose restriction enzymes that...

7. Celebrate National Book Month with Addgene

   Type

   Blog Post

   Published

   Oct. 3, 2019, 12:47 p.m.

   ...diverse perspectives. Everyone should read this book! Bonus, Saini's following book Superior: The Return of ...

8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   Published

   May 21, 2019, 12:10 p.m.

   ...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

9. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   Published

   April 7, 2022, 1:15 p.m.

   ...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ...

10. Trading Spaces - Visiting Scientist Edition

    Type

    Blog Post

    Published

    Aug. 22, 2023, 1 p.m.

    ...acquire the data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new...

11. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...attention and answer my key questions. As an added bonus, I also got the opportunity to present what I learned...

12. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type

    Blog Post

    Published

    June 1, 2021, 1:15 p.m.

    ...helped tomorrow. Our activity is non-profit and pro bono. If this sounds appealing, join us and help make...

13. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...

14. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019....

15. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...

16. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...isotypes, such as the number and location of disulfide bonds, the number of constant domains, and the hinge region...

17. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ...different wavelengths. Addgene provides empty backbones with the luciferase gene, a variety of expression...

18. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ...communication skillsClear communication is the backbone of effective management. Miscommunication often...

19. Viral Vectors 101: Viral Vector Elements

    Type

    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...plasmids for their production. As such, there are bonus components to these vectors that aren’t reflected...

20. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with...