We narrowed to 182 results for: cre encoding plasmid

... diverse secretory routes in various conditions. The plasmids and lentiviral vectors encoding these proteins...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...tRNA/aminoacyl-tRNA synthase pair is encoded on a single plasmid and facilitates the incorporation of ... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...

...apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters. Several...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence 10-fold after protease activity (... (To et al., 2016). However, the lab wanted to increase the fluorescence range to better study the spatio-temporal...β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters...

...clone the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure...changes in the nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide....Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

...towards successful iEP reprogramming. MettI7a1 encodes an uncharacterized putative methyltransferase. ... on Addgene.org Visit our stem cell page Find plasmids for reprogramming and differentiation Check out...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...

...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their ... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...them. HRP RANbody staining results in a ≥10-fold increase in signal over staining with a primary/ labeled...

...Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...to bind to a previously hidden epitope (right). Created withBioRender.com.   Biological Processes  ...

...cells to retain plasmids - if a cell does not inherit plasmid due to improper plasmid partitioning, it...unstabilized plasmids were retained in less than 10% of cells while plasmids containing the plasmid addiction...selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF contains...ensure that plasmids are passed down through generations (Figure 1). These so called “plasmid addiction ...only survive if they retain the plasmid. Since their discovery, plasmid addiction systems have been used...found that if a mini-F plasmid carried a specific 700 bp segment of DNA, the plasmid was propagated better...restriction-modification systems onto plasmids allowed cell populations to retain more of these plasmids as compared to those...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode, and...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds...libraries. Each mRNA molecule in the library doesn’t encode a stop codon, which causes the ribosome to pause...efficiently subcloned into the phagemid pDX_init plasmid, using the fragment exchange (FX) cloning technique...

...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...number of target cells that are positive for vector encoded transgene expression following transduction with...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

...Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB... tnsB, tnsC, tnsD, and tnsE), and a plasmid that contains the Mobile CRISPRi cassette flanked by left ...copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient cells during a tri-parental...Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred to recipient...transposon containing the CRISPRi components and a plasmid with the Tn7 transposition genes are transferred...RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the ability to synthesize diaminopimelic...

...performed by transfecting cells with a plasmid, such as PX459, which encodes Cas9 and contains a site for inserting...chosen for both Cas9 and gRNA expression       Plasmid DNA may be incorporated into the genome     Off-target...targets with similar efficiency as compared to plasmid-based expression of Cas9/gRNA and can be used for... as CMV or EF1A promoters found in many CRISPR plasmids) are not expressed. Because this method does ...fluorophore tagged dCas9 may require the use of plasmid or viral-mediated delivery. You’ve decided to...and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab...experimental systems that may not be amenable to plasmid based methods. One of the most common techniques...

...the endogenous target, and thus no genetically encoded tag is required. These dyes can also be attached... HaloTag®. These tags consist of a genetically encoded enzyme variant that covalently reacts with a small...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...Self-Labeling Protein Tags Fluorescent protein plasmids and resources A practical approach to choosing...fluorescent dyes, and fluorescent ligands. Figure created with BioRender.com. Fluorescence refers to...fluorescent protein tags, such as high specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

...packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid which provides...AAV plasmid standard and AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not... viral replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged ...going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs which are...cultures of your AAV transfer plasmid at 30 °C instead of 37 °C and then screen for ITR recombination with...to miniprep 5 mL of my AAV transfer plasmid cultures and then screen with SmaI digests. I freeze the bacterial...AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate...

... Resistance Gene Plasmids 101: Yeast Vectors Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter...blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection Plasmids 101:...species. Created with BioRender.com. How are shuttle vectors different from other plasmids? If we all...molecular code, can I transfect any old bacterial plasmid into HeLa cells and get on with my experiment? ..., where DNA replication begins, so a bacterial plasmid needs a compatible ori that will be recognized ...prokaryotic growth. Since there are no natural mammalian plasmids, mammalian vectors are even less similar to bacterial...transfection. In stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so...

...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...scientists through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the...

...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent...cloning your protein of interest into a vector encoding a fluorescent tag. Once expressed as a fluorescent... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...

...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...Once the recipient cells take up the Cre-tagged mitochondria, Cre is released, translocates to the nuclease...future improvements. Find PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024)....cell types, and tissues. Find cell-surface HRP plasmids here! Wu, L., et al. (2025) The cell-surface ...mitochondria (Hoover et al., 2025). MitoTRACER relies on a Cre-lox switch to turn recipient cells from red to green...systems, both in vitro and in vivo. Find MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023... FRCaMPi By Mike Lacy Many popular genetically encoded calcium indicators (GECIs) are available, but red...

...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...target site by the pegRNA — a guide RNA that also encodes the desired edit and homology to the genomic DNA...require multiple steps and enzymes. Prime editing can encode any of the 12 possible base-to-base changes in ...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration..., the Liu lab created and evaluated 19 PE1 variants with RT mutations known to increase activity, enhance... initial nick site (to avoid creating a double strand break), increasing editing efficiencies 2-3-fold...

...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome...(Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the endogenous MECP2 gene...excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet 23:185...two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging their combined...