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We narrowed to 535 results for: epo

Showing: 121 - 140 of 535 results

  1. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already... labs all around the world. We thank our many depositors for making these reagents available to the research...

  2. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

  3. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...with scientists, but 2012 was marked by increased deposits (and requests) of biosensors, optogenetics, and...

  4. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...using the BE4max architecture. The new editors reported in this study have ~10-100-fold lower average ... gRNA multiplex. Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  5. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes....ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be available...available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request service...ensuring we can maintain high standards for both our depositors and requestors.  Requesting Plasmids available...

  6. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...behaving mice to gather the following types of data: Report time-course and concentration of released DA in...optogenetically activated and inhibited neurons in live mice Report modulation of DA signaling during learning and ...moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...looming stimulus in vivo in transgenic zebrafish Report DA release in live mice over the course of Pavlovian...

  7. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new .... This saves us time as we are often analyzing deposits with many plasmids that contain similar features...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  10. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...typical entry level tasks such as stuffing new depositor welcome kits, note taking for protocol video production...

  11. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges of creating...addition to the poster session was a first for our repository, and I was proud to be responsible for sharing...numbers of plasmid genome engineering tools – both deposited and distributed – have changed so dramatically...

  12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids.... Anna Pyle’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase...

  13. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is...did you use? For animal subjects it is helpful to report the genetic background and catalog number or RRID...take negative data, which is equally important to report. We recently published a paper in Frontiers in ...looking for ways to improve the viewing or data deposition experience. If after submitting or viewing the...

  14. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...the reach and impact of your work. Please email deposit@addgene.org if you would like to contribute to ...University of California Davis School of Medicine. This deposit includes frequently cited antibodies for use in...reagents, share your plasmids via Addgene! Email deposit@addgene.org to learn more or get started.  If you...

  15. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

  16. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX.(cyto...new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

  17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

  18. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ..., the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/...While the Nuffield Council has recently published a report on the social and ethical issues of genome editing...question of “how do we move forward” is the June 2012 report on the ethics of “Novel techniques for the prevention...diseases. The key success of the Nuffield Council’s report is that when it became clear that this assistive...

  19. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...look back at some of the contributions from our depositors. Below are the 15 top requested plasmids over...2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and contains an envelope... the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE -... Thank you! We would like to thank all of our depositors and requestors for making their reagents available...

  20. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Listen to the hot plasmids... In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed evolution...
Showing: 121 - 140 of 535 results