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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik...manipulate precise proportions of cells. bioRxiv 788679; https://doi.org/10.1101/788679 Lin R, Wang R, Yuan J, ...of single neurons. Nature Methods 15:1033–1036 . https://doi.org/10.1038/s41592-018-0184-y Additional ...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....sparse labeling Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter...activity here Diessl et al., Microbial Cell, 2020. https://dx.doi.org/10.15698%2Fmic2020.04.713 Truncated...expressing tTALE-FP Shin et al., Sci. Rep. 2019. https://doi.org/10.1038/s41598-019-53722-0 ... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....multiplex immunolabeling of brain. eLife 8:e43322. https://doi.org/10.7554/elife.43322 Bandrowski, AE, Martone...Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030 Bradbury... antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a Resources We would also... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586-021-03471-w Garcia-Beltran...disease severity and survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran...vaccine-induced humoral immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Additional... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Maynard LH, Smith...Settings. Human Gene Therapy Methods 30:195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha... Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016/j.ymthe.2017.04.001 Additional... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....Antibody Staining. J Histochem Cytochem 62:197–204. https://doi.org/10.1369/0022155413511620 Lu QL, Partridge...Tissue Sections. J Histochem Cytochem 46:977–983. https://doi.org/10.1177/002215549804600813 Tuson JR, ...Histochem Cytochem : Off J Histochem Soc 38:923–926. https://doi.org/10.1177/38.7.2355173 Additional resources... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....in Medicine & Biology Society (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S...interfaces. Chemical Society Reviews, 48(6), 1826–1852. https://doi.org/10.1039/C8CS00710A Sahasrabudhe, A., Rupprecht...brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833-5 Resources on... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....have they done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511...Virus Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor...(pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi.1006508 Vicens Q,...Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi.0030044 Wuchty S,...Production of Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099 ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....Acids into Proteins. Science, 244(4901), 182–188. https://doi.org/10.1126/science.2649980 Shandell, M. A...and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/acs.biochem.1c00286 Zhu, P., Mehl...Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc.4541 ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....Frontiers in Cell and Developmental Biology 6: . https://doi.org/10.3389/fcell.2018.00054 Sander JD, ...targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard...Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722 Additional resources... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....throughout the year. Find workshops in your area here: https://comscicon.com/. All our events are offered free...fantastic K12 education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing...writing collaboratives like Oceanbites (https://oceanbites.org/) have already grown out of our workshops... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....Prospects of Antibody Fragments. Antibodies 8(2):28. https://doi.org/10.3390/antib8020028 Wessel GM, McClay...Histochem Cytochem : Off J Histochem Soc 34(6):703–706. https://doi.org/10.1177/34.6.3084626 Zhang Q, Miyamoto...super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016/j.crmeth.2022.100301 Additional... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....possible. Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet...Goudeau et al., 2020. Goudeau, J. bioRxiv 2020. https://doi.org/10.1101/2020.07.02.185249 Predicting ...proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804 The CRISPR... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m..... 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 Erickson, P...Methods in Cell Biology (pp. 283–310). Elsevier. https://doi.org/10.1016/s0091-679x(08)60255-1 Manning..., p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0038313 More resources... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....Application to DNA Assembly. ACS Synthetic Biology. https://doi.org/10.1021/acssynbio.8b00333 Pryor, J. M....Design. ACS Synthetic Biology, 11(6), 2036–2042. https://doi.org/10.1021/acssynbio.1c00525 Pryor, J. M....data-optimized assembly design. PLOS ONE, 15(9), e0238592. https://doi.org/10.1371/journal.pone.0238592 ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....Hackerspace Website https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org...www.openworm.org/ Cat Microbiome Project Website: http://www.kittybiome.com/ ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018-0368-2 Liu X, Zhang...by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen...chromatin by biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional ... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003 Guerin K, ...Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire...Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia...