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We narrowed to 256 results for: human dat

Showing: 121 - 140 of 256 results

  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...viral vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://...Infectious Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org...at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet for AAV Quantification... the absence of unexpected DNA contaminants. The data is also used to determine recombination rates in... vivo for gene expression and/or function. These data are sometimes reported or posted on the material...supernatant is tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination...written by multiple Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references...

  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...mammalian cells, but mammalian cells can perform human-like post-translational modifications that bacterial...production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster...performed during your harvest. Validation Recombinant antibodies should be validated to ensure they bind their...antigens specifically. Possible applications for validation include western blot, immunohistochemistry, and...produced, harvested, purified, quantified, and validated, it’s ready for use! You can learn more about ...

  3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ... along with other viruses that cause disease in humans such as measles, mumps, and rabies. Research on...for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome...VP30, and L (reviewed in 5). The RNA genome is encapsidated by the nucleoprotein, NP. NP associates with...mRNAs are translated) by cellular machinery. NP encapsidates the negative sense, single-stranded RNA minigenome...viral polymerase complex then recognizes the encapsidated minigenome as a template for replication and...

  4. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ... reveals specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. (2016). PubMed PMID: 27272384...Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells." Nat Biotechnol. (2016). PubMed PMID: 27347757...Updated Aug 13, 2020. Note: Cpf1 is also called Cas12a. In 2015, Zetsche et al. added to the CRISPR ...activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found two that display robust...publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification of CRISPR-Cas systems...

  5. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...whether the client invests substantial monetary and human resources towards the development of a new therapy...the business of life sciences and it's impact on human health.     Resources on the Addgene Blog Learn... and help resolve it through well-informed recommendations. Healthcare consulting firms that specialize...designing a strategic vision for a portfolio of candidate drugs. A decision like that would allow the client...especially helpful since they both provide up-to-date information about the industry, recruiting processes...

  6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...adenovirus in mouse; HIV1, West Nile, and Influenza in humans), LY6A lacks a defined role in viral susceptibility...you’re interested in how PHP.B performed in non-human primates, check out these two references: Dayton...PHP.B to reach the CNS; yet LY6A is an unlikely candidate. It was discovered as an antigen upregulated on...this stretch of DNA, Ly6a seemed the most likely candidate due to its strong linkage to the PHP.B phenotype...variants could unintentionally limit the utility of candidate capsids.  Are you interested in using AAV-PHP....

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...whether or not your study is aimed at developing human therapies. If a therapeutic is the end goal, it ...that your results are viable for translation to human patients. Picking receptors The receptor you choose...written by Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...happens within the first six hours. When added to human cells six hours after the introduction of Cas9 RNPs...Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute...during integration of transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-...check out our viral vector guide pages to get up-to-date tips for your virus experiments. References 1....

  10. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for ...R. K., Doudna, J. A. Enhanced homology-directed human genome engineering by controlled timing of CRISPR... to identify knock-in clones by reading up on validating genome edits to find screening strategies for...

  11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...generate in-house) a pre-validated antibody for each target protein. To study 20,000 human proteins, this would... a minute. As of now, the tool is available for human and mouse genomes only and deals with C-terminus...Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent tagging...In the light of these results, we re-analyzed our data and found that 75% of our highest knock-in efficiency...

  12. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...specify what species it is. Example: ‘h’ is for Human (homo sapiens), ‘m’ is for mouse (mus musculus),...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... R. J. (2022). AAV vectors: The Rubik’s cube of human gene therapy. Molecular Therapy, 30(12), 3515–3541... virus as a delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy... AAV can be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have...

  14. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...viral vectors and improved alternative methods." Human Gene Therapy, Part B: Methods 23.1 (2011): 1-7. ...adeno-associated virus type 2 Reference Standard Material." Human gene therapy 21.10 (2010): 1273-1285. PubMed PMID...concentration. We regularly make and validate fresh plasmid standards. We validate our absolute quantification...preparations. Addgene has used this method to generally validate our viral vector preparation protocol, and we ...around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010). In addition...targets: from variable nonsense to publication quality data." Scientific Reports 7 (2017). PubMed PMID: 28546538...

  15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...scientists to identify the proteomic map of the human mitochondrial matrix (Rhee et al., 2013) and determine...ligase (Kim et al 2016). BioID2 consists of a humanized biotin ligase from A. aeolicus, which lacks the...proteins is hard to imagine yet alone study. To elucidate these interactions scientists have long used antibody...labeling (Rhee et al., 2013). APEX catalyzes the oxidation of biotin-phenol to the short-lived biotin-phenoxyl...complementation (PCA) technique with proximity labelling to validate binary protein-protein interaction as well as ...Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through Spatial Proximity...

  17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep...Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https://doi.org/... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...) is the smallest Cas9 ortholog characterized to date. Kim et al. successfully used CjCas9 with AAV to...

  18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome...reverse transcriptase with a sequence optimized for human codons; additional nuclear localization sequences...of heteroduplex     ✓ ✓ ~ ~ ~ Human codon optimization Improve prime editor expression...Traditional sgRNAs are protected from cellular degradation by Cas9 binding, but pegRNAs have an extended... written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December 2024. References...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...the potential CRISPR technology can have on the human microbiome. You can consider the microbiome as a...and metabolic processes, occupying the gut. Many human diseases have connections to either specific organisms.... Engineering the bee gut microbiome Beyond the human gut, the Barrick lab has deposited plasmids that...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...may be functional in most Enterobacteriaceae. An update to this system, called pEcCas/pEcgRNA, allows it...addition of a uracil DNA glycosylase inhibitor and degradation tag on the fusion protein to limit the activity...

  20. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...Advancement of Science) believe GMOs are safe for human consumption in comparison to 37% of the general ...scientists believe climate change is mostly due to human activity while much less of the general public agrees...help support or reject a hypothesis. Looking at the data, scientists then revise their hypotheses and the... be useful to governments, however, inconvenient data must not be ignored. Returning to an earlier analogy...lose trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust...pandered to when strong or overlooked when small, but data is not going change depending on the number of people... as I believe people that can see and understand data themselves can come to conclusions themselves instead...
Showing: 121 - 140 of 256 results