We narrowed to 141 results for: ipp
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TypeBlog PostPublishedFeb. 17, 2022, 2 p.m.... are as bad at integrating as they are good at flipping. So the lab instead turned to phage serine integrases...
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Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....µm. C) Images of iPAK4 fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....analysis. I'm often asked whether this step can be skipped or whether selecting colonies and performing Sanger... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....manipulate phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m.... In addition, PVDF membranes can be repeatedly stripped of an antibody and reprobed with different antibodies... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....missing or added at the broken ends and, as such, cripple the gene. However, if repair material is provided... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m.... Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K (2014... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon... -
9 tips for a successful postdoctoral experience
TypeBlog PostPublishedOct. 9, 2018, 12:21 p.m....did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....member who knows how the physical kits are made and shipped; Chiara, the tech transfer expert who understands... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon imaging of natural... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for selective... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....destabilizing the base pairs closest to the PAM and flipping them out of the DNA duplex to pair with the guide... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....in ΔΔCT are represented by RQs between 0 and 1. Flipping your experimental and control values during analysis... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. ...