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Showing: 121 - 140 of 143 results

  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based) Mammalian Lentivirus pRDA_722...lentiCRISPRv2-based; preferred over 512) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian...Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector...

  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  3. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...

  5. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  10. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  19. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...production process.  Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize...
Showing: 121 - 140 of 143 results