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We narrowed to 123 results for: lentiviral vector

Showing: 121 - 123 of 123 results
  1. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: 10074136... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...
  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, ...Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...
  3. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...
Showing: 121 - 123 of 123 results