Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 474 results for: nol

Clear keywords
Showing: 121 - 140 of 474 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...

  2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...variety of research sectors to provide open source technologies and spaces where interested parties can do research...is a passion for learning and having fun with technology, so feel free to contact your local space and...Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow Derek on Twitter @derekja...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology...discovery through the creation of innovative technologies. To date his group has develop several popular...catalytically active Cas9 nuclease." Nature biotechnology (2015). PubMed PMID: 26436575. PubMed Central...

  5. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...indel formation (gene disruption) with this new technology. To dig more into what researchers felt about...genome editing with NgAgo, this does not mean the technology is dead in the water. On the bright side, successful...Natronobacterium gregoryi Argonaute." Nature biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...metabolic cassettes (Zhang et al. 2020); however, the technology potential enabled by CRISPR-transposons has yet...CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter @Vopinator...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...the production of recombinant Erythropoietin. J Biotechnol. 2020 Feb 10;309:44-52. doi: 10.1016/j.jbiotec...beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002...

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...approximately 20,000 droplets using water-oil emulsion technology. Each droplet contains the ingredients for amplification... added benefit, replicates are built into the technology. One well (containing thousands of droplets) ...titration, there are many applications for the technology.  Droplet digital PCR is well suited for the ...

  9. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ... CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID: 27918548. ...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids...CRISPR-Cas Cpf1 nucleases in human cells." Nat Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids...

  10. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin...at the Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa...

  11. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587...with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587...and C-to-G editing in mammalian cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...showed APEX2 can use an orthogonal substrate, alkyne-phenol, to label proteins with an alkyne group for click... tapes for intracellular recordings. Nature Biotechnology, 41(5), 631–639. doi: 10.1038/s41587-022-01524...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

  14. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...impact (15). Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This... 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern research,...Trump’s first budget Environmental Science and Technology: Crossing The Imaginary Line The New York Times...

  15. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...David Liu’s lab by combining Cas9 DNA-targeting technology with a fused cytidine deaminase to create C→T...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...Cas domain compatibility and activity. Nature Biotechnology, 38(7), 883–891. https://doi.org/10.1038/s41587...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...science, IPI hosts events and training courses on technologies and laboratory techniques related to antibody...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...avidin-biotin technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 ...interaction with avidin for molecular biology and biotechnology purposes.  Once a polypeptide is biotinylated...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...especially in in vivo models, and new Cre-lox based technologies are still being developed today. In this post...name Brainbow is truly fitting for this colorful technology. Optimizing the system: Brainbow-3 Although Brainbow... et al. released a refinement of the Brainbow technology, Brainbow-3.0, with the goal of overcoming the...

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...They also have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase...fluorescent proteins so you can best apply these technologies to your own research needs. Other companies ...series of blog posts on the defunct genome editing technology NgAgo). In addition, there are organizations ...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007/s12033-008... on efficacy, safety and upstream production. Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/...seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002...
Showing: 121 - 140 of 474 results