We narrowed to 397 results for: organization Addgene

...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on the...

...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific...

...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...

...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...plasmids or plant expression systems available from Addgene. If you have any questions or thoughts about this...PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed....  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI nucleases for..... Find the plasmids from this publication at Addgene ...

...in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...excellent questions.    Additional Resources on the Addgene Blog Using virus in your research - a primer for...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised... Turnover at Addgene is about half of what is standard in most sectors. The Addgenies support a high... ahead of the curve on this. Addgene’s success is about the Addgenies. I never worry about their dedication...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...

...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and...

...handful of Click Biology tools available at Addgene. Addgene Link Click Biology Tag Description Humberg...already deposited at Addgene in multiple formats: for use in different organisms, targeted to particular... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...by dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring stable ...signaling. Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding ...s41589-025-01944-x   Additional Resources on the Addgene Blog Live and Let Dye: Self-Labeling Protein Tags...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...

...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing... Various Addgenies...

...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Services  Resources on the Addgene blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...shipped 2,078 items to 764 scientists at 375 organizations in 21 different countries. It's safe to say ...publication by pasting the paper's title into the Addgene website search bar?   We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...

...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...based biosensors.     Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be.../10.1002/iub.165.  Additional resources on the Addgene Blog CRISPR libraries Genome-wide screens with...

...Additional Resources on the Addgene Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

...to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists share...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...