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We narrowed to 440 results for: pes;

Showing: 121 - 140 of 440 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium...-specific targeting. By employing various AAV serotypes, researchers can precisely direct the calcium ...sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream monitoring...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging...prebuilt donor plasmid library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent...proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II,...

  3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, second chromosome...stubble (sb). For a better reference of various fly phenotypes, check out the Classical Genetics Simulator website...whether stocks of mutant flies exist, whether any phenotypes have been observed from mutations in the gene...

  4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ... is transient expression sufficient? Which cell types will you be editing? Do you want to deliver the ...cell lines. Useful for difficult to transfect cell types and can be used in vivo. A common choice for conducting...Electroporation / Nucleofection Microinjection Cells Many types Single cells Cargo Nucleic acids or protein Nucleic...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... of cells as starting material. Therefore, cell types that are of low abundance are not particularly well... controls, multiple replicates and several cell types can strengthen your results. Enrichment or depletion... bit about how CRISPR/Cas9 can be used in other types of experiments, check out our CRISPR Plasmids and...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...on the search page to quickly jump to different types of items: Did you notice the “Filter By…” options...The search bar will suggest direct links to other types of searches including depositing labs and institutions...can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of...

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or... For detailed protocols, volumes, spin speeds, recipes, and Agrobacterium strains see Weigel and Glazebrook...occurs in a coding region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate...

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ... multi-gene knockouts in a wide variety of cell types, gene editing using homology directed repair (HDR...internalization of Cas9-gRNA RNPs by specific cell types (Rouet et al. 2018). This was accomplished using...additional factors to take into account with these types of experiments. One important consideration is that...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally...naturally occurring and engineered AAV serotypes that further expands the range of AAV properties is available...available. While only natural serotypes are currently used in clinical trials, a variety of engineered ones...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some ...Addgene’s repository is smaller than for other vector types, we hope that this introduction gives a sense of...Production and Troubleshooting Viral Vectors 101: Types of Viruses...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...majority of cellular repair events will generate these types of mutations, making it an ‘easy’ editing job to... DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly...about optimizing this distance. Homology arms and types of donors To make sure your donor molecule is used...

  12. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...strategies available for assembling these modular types of constructs, and different methods are suited ...specific restriction enzymes used affect what general types of modules can be assembled. For example, because... MXS-chaining method can be used to build these types of constructs robustly, quickly, and simply. We ...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...groups and departments. You don’t have to get these types of projects and groups going alone - if you are ... discipline. Getting companies to come to these types of events can be very tough. First I needed to find...growth through my interactions with many different types of people. As mentioned in the beginning, the ability...

  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...repressing target genes in a variety of species and cell types can be found on Addgene’s website. Activating target...activating target genes in a variety of species and cell types can be found on Addgene’s website. Fig. 3...optimized for expression in different species and cell types including (but not limited to) bacteria, yeast, ...

  16. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...cell types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia...applied to the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... a gene in a living animal across multiple cell types using a mouse model for a human neurological disease...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed... improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002). Neurology 92 (15 Supplement...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...surface, expanding the range of targetable cell types. DIRECTED is compatible with fusogens from different...such as lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible...Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology...

  19. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene, including cDNA...Addgene are lentiviral pooled libraries, though other types exist.  When you buy a pooled library from Addgene...Spinfection transduction efficiency can vary across cell types and generally requires optimization of many variables...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...revolutionize neuroscience Manipulation of specific cell types in neuronal networks Double inverted vectors expressing...only means to target and manipulate specific cell types (populations) within neuronal networks in living...Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping.” Journal...
Showing: 121 - 140 of 440 results